·糖尿病基础研究·

　　【摘要】 目的 观察螺内酯对T2DM大鼠脂肪组织脂联素、PPARγmRNA表达的影响。 方法 高脂饲养加小剂量链脲佐菌素注射建立T2DM大鼠模型。雄性SD大鼠50只，分正常组(NC,n=10)，糖尿病对照组(DM-C，n=15)和糖尿病干预组(DM-S,n=14)，实验末留取血液和附睾脂肪组织，检测血液学指标和脂肪组织脂联素、PPARγ的表达水平。 结果 DM-C组和DM-S组血TG均高于NC组(P<0.01), DM-S组水平低于DM-C组(P<0.05)。脂肪组织中脂联素和PPARγmRNA表达，DM-C组低于NC组[(4.26±0.5) vs (4.85±0.24)，(5.12±0.28) vs (5.43±0.22),P<0.05],DM-S组高于DM-C组[(4.72±0.33 )vs (4.26±0.5)，(5.36±0.22 )vs (5.12±0.28),P<0.05]。 结论 螺内酯上调T2DM大鼠脂肪组织中脂联素及PPARγmRNA的表达,降低血TG，这可能是改善糖尿病IR和靶器官损伤的一个潜在机制。

　　【关键词】 糖尿病，2型;螺内酯;脂联素;PPARγ;脂肪组织

Pironolactone influences the expression of adiponectin and PPARγ in adipose tissue of type 2 diabetic rats WAN Hong-mei, HAN Gang, SUN Kan, et al. Medical College of Shihezi University, Xinjiang 832000, China

Corresponding author: HAN Gang, E-mail: hangang0805@sohu.com

　　【Abstract】 Objective To investigate the effect of spironolactone on the expression of adiponectin and peroxisome proliferator activated receptor gama (PPARγ) mRNA in epididymal adipose tissue of type 2 diabetic rats. Methods The model of T2DM rats were established through high-sucrose-high-fat diet and followed by low dose Streptozotocin injecting. Fifty healthy male Sprague-Dawley rats were divided into 3 groups: Normal control rats (NC), diabetes control group (DM-C), and diabetic rats treated with Spironolactone (DM-S). The group DM-S was treated with Spironolactone via intragastric administration for 8 weeks. Serum markers were tested and the mRNA expression of adiponectin and PPARγin adipose tissue were detected. Results Triglyceride level was significantly higher in group DM-C and DM-S compared with group NC (P<0.01) and it was lower in group DM-S than in group DM-C (P<0.05). The mRNA levels of adiponection and PPARγ were lower in group DM-C than in group NC [(4.26±0.5) vs (4.85±0.24) and (5.12±0.28) vs (5.43±0.22), (P<0.05)], and were significantly increased in group DM-S as compared with group DM-C [(4.72±0.33) vs (4.26±0.5) and (5.36±0.22) vs (5.12±0.28), (P<0.05)]. Conclusion Spironolactone increases expression of adiponectin and PPARγ mRNA in adipose tissue and reduces serum TG level of type 2 diabetic rats, which may involve a potential mechanism for protection against insulin resistance and lessening tissue damage in diabetes.

　　【Key words】Diabetes mellitus, type 2;Spironolactone;Adponectin; Peroxisome proliferator activated receptor gama (PPARγ);Adipose tissue