·糖尿病基础研究·

　　【摘要】 目的 探讨Exendin-4对TNF-α诱导下的大鼠肾小球系膜细胞氧化应激和转化生长因子β1(TGF-β1)表达的影响。 方法 培养大鼠肾小球系膜细胞，分为对照(A)组、TNF-α(B)组、TNF-α+Exendin-4(C)组。培养48 h后，用分光光度比色法测定细胞上清液超氧化物歧化酶(SOD)活性、丙二醛(MDA)、谷胱甘肽(GSH)的含量，采用流式细胞术检测ROS含量，ELISA法检测TGF-β1蛋白表达。 结果 B组系膜细胞培养上清SOD (15.46±1.19) U/ml、GSH(18.48±1.16) μmol/ml，均低于A组，差异有统计学意义(P< 0.01)。B组细胞培养上清MDA(4.56±0.26)nmol/ml、TGF-β1 (375±14) pg/ml，ROS平均荧光强度为(1100±40)，与A组比较，差异有统计学意义(P< 0.01)。Exendin-4干预可拮抗TNF-α刺激下系膜细胞的上述改变。 结论 Exendin-4可抑制TNF-α诱导的系膜细胞氧化应激和TGF-β1表达，提示Exendin-4可能会对DN有保护作用。

　　【关键词】 系膜细胞;氧化应激;转化生长因子β1;Exendin-4;大鼠

Effect of Exendin-4 on the oxidative stress and expression of TGF-β1 in cultured glomerular mesangial cells induced by TNF-α in vitro JIANG Ying-juan, XUE Yao-ming, ZHANG Yan-fei, et al. Department of Endocrinology and Metabolism, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou 510515, China

Corresponding author: XUE Yao-ming, E-mail: xueyaoming999@126.com

　　【Abstract】 Objective To investigate the effect of Exendin-4 on the oxidative stress and expression of TGF-β1 in cultured glomerular mesangial cells induced by TNF-α in vitro. Methods The cultured mesangial cells were divided into control group, TNF-α group and TNF-α+exendin-4 group. After 48h, the supernatant was collected. The activity of SOD, the content of MDA, GSH, and ROS, and the expression of TGF-β1 were detected by spectrophotometry, flow cytometry and ELISA respectively. Results Compared with the control group, the activity of SOD (15.46±1.19)U/ml and the content of GSH (18.48±1.16)μmol/ml in the TNF-α group was found to be significantly decreased, while the content of MDA (4.56±0.26)nmol/ml and ROS (1100±40), and the expression of TGF-β1 (375±14)pg/ml increased, the differences were statistically significant (P<0.01). Exendin-4 could inhibit the changes of the mesangial cells (P<0.01). Conclusion Exendin-4 can reverse the effect of TNF-α on the oxygen stress and expression of TGF-β1 in mesangial cells. The reasonable application of exendin-4 may be helpful in preventing and treating diabetic nephropathy.

　　【Key words】Mesangial cells; Oxidative stress; Transformation growth factor (TGF-β1); Exendin-4; Rat