糖尿病分子生物学和遗传学

　　【摘要】 目的 探讨7个单核苷酸多态性位点(SNP)与T2DM的关系。 方法 采用高分辨熔解曲线(HRM)检测T2DM组202例和健康对照(NC)组200名的7个易感基因位点，分析这些易感基因位点多态性与T2DM的相关性。 结果 两组rs8050136、rs13266634、rs7578597、rs864745和rs7961581位点比较差异有统计学意义(P<0.01)，rs8050136、rs13266634、rs7578597和rs864745位点OR>1，rs7961581位点OR<1。两组rs10811661、rs10923931位点比较差异无统计学意义，OR均接近于1。 结论 rs8050136、rs13266634、rs7578597和rs864745位点多态性与我国汉族人群T2DM发病风险相关，rs10811661、rs10923931位点多态性与我国汉族人群T2DM发病风险无相关性，rs7961581位点多态性对我国汉族人群T2DM可能起保护作用。

　　【关键词】单核苷酸多态性;糖尿病，2型;高分辨熔解曲线

　　【Abstract】 Objective To investigate onthe correlation between the seven single nucleotide polymorphisms and genesis of T2DM. Methods High-resolution melting (HRM) curve technology was used to detect thegenotype of the seven SNPs in 202 T2DM patients and 200 healthy volunteers. The relationship between susceptible gene polymorphism and T2DM was analyzed. Results The frequency of five SNP loci rs8050136, rs13266634, rs7578597, rs864745 and rs7961581 showed significant differences between T2DM patients and controls (P<0.01). The OR of rs8050136, rs13266634, rs757859 and rs864745 was >1, while OR of rs7961581 was <1. There was no significant difference in rs10811661 and rs10923931 loci between T2DM patients and controls (OR=1). Conclusion The polymorphism of loci rs8050136, rs13266634, rs7578597 and rs864745 is associated with T2DM genesis in this study population, while rs10811661 and rs10923931 is not associated with T2DM genesis, and rs7961581 might be a protective factor for T2DM.

　　【Key words】Single nucleotidepolymorphisms (SNPs);Diabetes mellitus，type 2;High-resolution melting (HRM) curve