【摘要】 目的 观察微小RNA(miRNA)-146a转染H9c2心肌细胞高糖培养下NF-κBp65和Ι型胶原表达。 方法 高糖培养H9c2细胞系，分为增强组(EC)、阴性对照组(NC)、空白对照组(BC)。采用Lipofectamine 2000 Transfection Reagent分别转染miRNA-146a增敏剂(50 nmol/L)、错义链(50 nmol/L)、PBS，于48 h后Real-time PCR测定miRNA-146a水平，Western-blot测定NF-κBp65和Ⅰ型胶原水平。 结果 转染后，EC组H9c2细胞miRNA-146a水平高于NC组和BC组(P<0.05)，NF-κBp65和Ⅰ型胶原蛋白表达量低于NC组和BC组(P<0.05)。 结论 miRNA-146a参与了高糖培养H9c2心肌细胞纤维化，其机制与NF-κBp65和Ι型胶原表达有关。

　　【关键词】 心肌细胞;miRNA-146a;NF-κBp65;Ⅰ型胶原

Expression of NF-κBp65 and collagenⅠof H9c2 cell transfected by microRNA-146a under high glucose culture XU Qiu-ling, JIN Xiu-dong, ZHANG Xu-dong, et al. School of Basic Medicine，Heilongjiang University of Chinese Medicine ,Harbin 150040, China

　　【Abstract】 Objective To investigate the expression of NF-κBp65 and collagenⅠin high glucose-cultured H9c2 cell transfected by miRNA-146a. Methods The high glucose-cultured H9c2 cells were divided into enhanced group(EC), negative control group (NC) and blank control group(BC). Three groups were transfected by a synthesized miRNA-146a mimic , PBS and lipofectamine 2000 respectively. After 48 h ,the miRNA-146a expression were detected by real-time RT-PCR, the NF-κBp65 and collagenⅠ expression were detected by Western blot. Results After transfection, the miRNA-146a expression were obviously higher in EC group than in NC and BC group(P<0.05). The NF-κBp65 and collagenⅠ expression were significantly decreased in EC group than in NC and BC group(P<0.05). Conclusion miRNA-146a is involved in fibrosis of high glucose cultured H9c2 cell. Its mechanism is related to the expression of NF-κBp65 and CollagenⅠ.

　　【Key words】 Myocardial cell; MicroRNA-146a; NF-κBp65; CollagenⅠ