【摘要】目的 观察吡格列酮对系膜细胞(MCs)氧化应激的影响，探讨其肾脏保护机制。方法 体外培养MCs，分为正常对照(NG)组，高糖(HG)组及吡格列酮干预 (P1、P2、P3)组。应用流式细胞仪检测细胞内活性氧(ROS)水平，对上清液中总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、丙二醛(MDA)进行检测。 结果 与NG组比较，HG组细胞内ROS水平增加，T-SOD、CAT活力降低，MDA含量增高(P<0.01);吡格列酮各干预组上述变化均受抑制(P<0.05)，且呈剂量依赖性。结论 吡格列酮可剂量依赖性的抑制高糖诱导的MCs氧化应激水平。

　　【关键词】氧化应激;吡格列酮;系膜细胞

Effects of pioglitazone on high glucose-mediated oxidative stress in cultured glomerular mesangial cells of rats WANG Shan, YE Shan-dong, SUN Wen-jia, et al. Department of Endocrinology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China

Corresponding author：YE Shan-dong，E-mai：ysd196406@163.com

　　【Abstract】Objective To observe the effects of pioglitazone on high glucose-mediated oxidative stress in cultured glomerular mesangial cells of rats. Methods Rat glomerular mesangial cells were cultured in the medium with normal glucose concentration (group NG),high glucose concentration (group HG) and high glucose in combination with different concentrations of pioglitazone (group P1,P2,P3). The intracellular reactive oxygen species (ROS) was detected with flow cytometer. The supernatant levels of total superoxide dismutase (T-SOD),catalese (CAT) and maleic dialdehyde (MDA) were determined. Results Compared with group NG, the levels of ROS and MDA increased, while T-SOD and CAT decreased in group HG (P<0.01).When cultured with pioglitazone,those changes mentionted above in group HG were improved in a concentration-dependent manner (P<0.05). Conclusion Pioglitazone can alleviate high glucose-induced oxidative stress in a concentration-dependent manner in glomerular mesangial cells.

　　【Key words】Oxidative stress;Pioglitazone;Mesangial cells (MCs)