【摘要】 目的 探讨辛伐他汀对糖基化终末产物(AGEs)诱导内皮细胞环氧化酶2(COX-2)表达的影响。 方法 体外以不同浓度的糖基化白蛋白(AGE-BSA)、辛伐他汀单独或联合作用于人脐静脉内皮细胞，检测细胞COX-2 mRNA的表达及培养上清液中前列腺素E2(PGE2)的含量。 结果 AGE-BSA诱导内皮细胞COX-2 mRNA的表达，作用呈剂量和时间依赖方式。辛伐他汀可降低AGE-BSA诱导的COX-2 mRNA表达及PGE2产物浓度。 结论 AGEs促进内皮细胞COX-2表达，辛伐他汀可减轻此作用。

　　【关键词】 糖基化终末产物;环氧化酶-2;辛伐他汀

The effect of simvastatin on advanced glycation end products(AGEs) induced cyclooxygenase-2(COX-2) mRNA expression in human umbilical vein endothelial cells(ECV304) LI Hui-juan,CHENG Xing-bo ,SHI Bi-min,et al.Department of Endocrinology,The First Affiliated Hospital Of Soochow University,Suzhou 215006 , China

Corresponding author:LU Yi-qun, E-mail: lihuijuan1979@sina.com

　　【Abstract】 Objective To investigate the effect of simvastatin on advanced glycation end products(AGEs) induced cyclooxygenase-2(COX-2) mRNA expression in human umbilical vein endothelial cells(ECV304). Methods ECV304 were incubated with different concentrations of AGE-BSA and simvastatin singlely and combinedly for different hours. And the mRNA expression of COX-2 and the supernatant content of PGE2. were tested. Results After treatment with AGE-BSA concentrations of 50,100,200 and 400 mg/L, the mRNA expressions of COX-2 were increased in a dose-dependent manner, (P<0.05).After treatment with 200 mg/L AGE-BSA for 6, 12, 24 or 48 h，the mRNA expression of COX-2 were increased in a time-dependent manner, (P<0.05). Incubation with simvastatin at 1, 10 and 100 μmol/L as Add-on Treatment to 200 mg/L AGE-BS markedly prevented the upregulation of COX-2 mRNA expression, which correlated with an decrease in PGE2 level( P<0.05). Conclusions AGEs induce the expression of COX-2 and PGE2 of endothelial cells in a dose- and time-dependent manner, these effects could be inhibited by simvastatin.

　　【Key word】 Advanced glycation end products;Cyclooxygenase-2; Simvastatin