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 二甲双胍对3T3-L1脂肪细胞瘦素、肿瘤坏死因子-α表达与分泌量影响的观察

来自:中国糖尿病杂志  编辑:岳杉 张艳红 耿厚法|点击数:|2013-06-24

  ·糖尿病基础治疗·


  【摘要】 目的 观察二甲双胍对3T3-L1脂肪细胞瘦素、肿瘤坏死因子(TNF)-α表达与分泌量的影响,探讨二甲双胍降低体重、改善脂代谢的作用机制。 方法 3T3-L1前脂肪细胞分化成熟后分别予不同浓度与作用时间的二甲双胍干预,采用RT-PCR法检测细胞内瘦素、TNF-α mRNA的表达,ELISA法测定培养基内瘦素、TNF-α的分泌量。 结果 二甲双胍抑制3T3-L1脂肪细胞瘦素、TNF-α的表达与分泌,呈时间与剂量依赖性。 结论 二甲双胍抗肥胖改善脂代谢的作用可能与改善瘦素、TNF-α抵抗状态有关。

  【关键词】二甲双胍;瘦素;肿瘤坏死因子-α;肥胖

Effects of metformin on the expression and secretion of leptin and tumor necrosis factor-α in 3T3-L1 adipocytes   YUE Shan, ZHANG Yan-Hong, GENG Hou-Fa, et al. Graduate School of Tianjin Medical University, Tianjin 300070, China

Corresponding author: BAN Bo,E-mail: banbo2011@163.com

  【Abstract】 Objective To observe the effects of metformin on the expression and secretion of leptin and tumor necrosis factor (TNF)-α in 3T3-L1 adipocytes, and to explore its mechanism in anti-obesity and lipid metabolism improvement. Methods The 3T3-L1 preadipocytes were cultured and differentiated into adipocytes, then incubated with metformin at different concentrations and durations. Leptin and TNF-α mRNA expressions were assayed by RT-PCR. The contents of leptin and TNF-α were detected by ELISA. Results Metformin functions to inhibit the leptin and TNF-α mRNA expressions and secretions in 3T3-L1 adipocytes in a concentration- and time-dependent manner. Conclusion The function of metformin in anti-obesity and lipid metabolism improvement may be related with its improvement in leptin and TNF-α sensitivity.

  【Keyword】 Metformin; Leptin; Tumor necrosis factor-α; Obesity

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