·糖尿病基础研究·

　　作者单位：510282广州，南方医科大学附属珠江医院内分泌科

　　钙非依赖型磷脂酶A2对过氧化氢诱导人胰岛微血管内皮细胞凋亡的影响

　　张振 蔡德鸿 杨力 孙嘉 陈容平 杨锐 陈宏 张桦

　　【摘要】目的研究钙非依赖型磷脂酶A2 ( iPLA2 )对过氧化氢(H2O2 )诱导的胰岛微血管内皮细胞(IMEC)凋亡的影响。方法实验分空白对照组、重组腺病毒空载体组 (Ad-GFP组)、Ad-iPLA2组三组进行，每组均接受100 μmol/L H2O2和500 μmol/L H2O2两个干预浓度，作用时间12 h，每组细胞做6个复孔(n=6);采用Hoechst33258核染色形态学观察、定量分析以及Annexin V-APC/P I双染色检测细胞的凋亡情况。结果空白对照组与空载体组比较，在H2O2浓度(100、500 μmol/L)中细胞凋亡率差异均无统计学意义(P均>0.05);iPLA2组在100 μmol/L H2O2浓度下凋亡率为(17.16±1.53)%，明显低于其他两组(P均<0.05);而在500 μmol/LH2O2浓度下iPLA2组凋亡率(46.18±2.12)%，明显高于其他两组(P均<0.05)。结论iPLA2在100 μmol/L H2O2浓度下可抑制IMECs的凋亡，而在500 μmol/L H2O2浓度下则促进IMECs的凋亡。

　　【关键词】过氧化氢; 磷脂酶A;胰岛;内皮细胞;细胞凋亡

　　doi:10.3969/j.issn.1006-6187.2012.05.018

　　Influence of calcium-independent phospholipase A2 on the H2O2-induced apoptosis of islet microvascular endothelial cellsZHANG Zhen, CAI De-Hong, YANG Li, et al. Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China

　　【Abstract】ObjectiveTo investigate the influence of calcium-independent phospholipase A2 (iPLA2) on the apoptosis in islet microvascular endothelial cells (IMEC) induced by hydrogen peroxide (H2O2).MethodsHuman IMECs were divided into 3 groups (n=6): the control group, Ad-GFP group, and Ad-iPLA2 group. They were treated by different concentrations of H2O2(100 and 500 μmol/L) for 12 h. The apoptosis detection was performed by morphologic observation and quantitative analysis of IMECs stained with Hoechst33258 and by Annexin V APC/PI staining by FCM in each group.ResultsThe comparison between the control group and Ad-GFP group showed that there was no significant difference in the apoptosis ratio (all P>0.05). The apoptosis ratio of Ad-iPLA2 group [(17.16±1.53)%] was lower than the other two groups (P<0.05) in H2O2 100 μmol/L, but it was higher [(46.18±2.12)%] than the other two groups (P<0.05) in H2O2 500 μmol/L.ConclusionThe iPLA2 may inhibit the apoptosis of IMECs induced by H2O2 100 μmol/L, but may promote the apoptosis of IMECs induced by H2O2 500 μmol/L.

　　【Key words】Hydrogen peroxide; Phospholipases A; Islets of Langerhans; Endothelial cells; Apoptosis