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C-fos诱导生长因子在胰腺再生中的重要作用

来自:中国糖尿病资讯网  编辑:editor|点击数:|2012-05-31

  ·糖尿病基础研究·

  基金项目:国家自然科学基金(81160098)

  作者单位:750004银川,宁夏医科大学(滕兆刚);宁夏医科大学总医院(魏军、范恒、李玉奎、王立斌)

  通讯作者:魏军,E-mail:lydiajunwei@hotmail.com.cn

  C-fos诱导生长因子在胰腺再生中的重要作用

  滕兆刚 魏军 范恒 李玉奎 王立斌

  【摘要】目的探讨c-fos诱导生长因子(Figf)对小鼠胰腺再生的影响。方法建立小鼠胰腺损伤模型,于术前、术后12 h、24 h检测血糖,48 h后收集剩余胰腺组织。经全基因组芯片分析、Q-PCR验证Figf发生高表达。构建Figf过表达质粒,转染胰岛内皮细胞(MS 1),36 h后ELISA检测上清培养液胰岛素分泌量的变化,Q-PCR检测转染后MS 1中Pdx1、Insulin1 mRNA表达水平。结果小鼠胰腺再生过程Figf mRNA表达上调。与未转染组、转染PcDNA 3.1组比较,转染Figf过表达质粒组胰岛素分泌量明显升高[未转染组(116.89±6.09)(P<0.01);转染PcDNA 3.1组(114.24±4.60)(P<0.01)],转染Figf过表达质粒组Pdx1、Insulin1 mRNA表达水平均明显升高(P<0.01)。结论MS 1中过表达Figf可使Pdx1、Insulin1 mRNA表达水平升高,增加胰岛素的分泌。Figf可能在胰岛β细胞再生中发挥着重要作用,是小鼠胰腺再生的关键基因。

  【关键词】c-fos诱导性生长因子;胰岛内皮细胞;胰岛素;胰腺再生

  doi:10.3969/j.issn.1006-6187.2012.05.017

  The important role of c-fos-induced growth factor in the pancreas regenerationTENG Zhao-gang, WEI Jun, FAN Heng, et al. Ningxia Medical University, Yinchuan 750004, China

  Corresponding author: WEI Jun, E-mail: lydiajunwei@hotmail.com.cn

  【Abstract】ObjectiveTo explore the role of c-fos induced growth factor (Figf) in the pancreas regeneration.MethodsMouse model with pancreatic regeneration were established by pancreatectomy. Pancreatic function loss and regeneration was monitored by time-coursed blood glucose testing after pancreatectomy. The levels of blood glucose were measured before and 12 h and 24 h after operation. Regenerated pancreas tissues were collected 48 h after pancreatectomy for RNA isolation. Gene expression was performed using mouse whole genome chips. Differently expressed genes were verified by Q-PCR. The target gene, Figf, was selected and cloned in expression plasmid. MS 1 cells cultured in vitro were transfected with the Figf constructs, and insulin secretion by the transfected cells was detected by ELISA 36 h after cell transfection. The mRNA levels of Pdx1 and insulin1 genes in transfected cells were tested by Q-PCR.ResultsFigf expression was significantly increased in regenerating pancreas. As compared with the untransfected and vector-transfected groups, the insulin secretion in Figf transfected cells increased significantly [(116.89±6.09) pg/ml in the untransfected group (P<0.01); and (114.24±4.60) pg/ml in the vector-transfected group (P<0.01)]. As compared with the untransfected and vector-transfected groups, the Figf over expression group expressed significantly higher mRNA levels of Pdx1 and insulin1 genes (P<0.01).ConclusionOver expression of Figf gene in MS 1 cells can elevate the expression of Pdx1 and insulin1 in mRNA levels, and increase the insulin secretion. Figf may be one of the key genes involoving in pancreas regeneration and plays an important role in β-cell regeneration.

  【Key words】 C-fos induced growth factor (Figf); MS 1 cells; Insulin; Pancreas regeneration

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