糖尿病基础研究

　　【摘要】 目的 观察补充富含n-3多不饱和脂肪酸的亚麻籽油对高脂诱导肥胖小鼠血糖和GLP-1水平的影响。 方法 将30只雄性C57BL/6J小鼠分为正常饲料(ND)、高脂喂养(HFD)和亚麻籽油(FOD)组，喂养8周后，行腹腔注射葡萄糖耐量试验(IPGTT)和胰岛素耐量试验(ITT)和GLP-1分泌试验。 结果 FOD组FPG、胰岛素低于HFD组(P<0.01)。IPGTT结果显示，FOD组120 min内血糖曲线下面积(AUC)低于HFD组(P<0.01)。ITT结果显示，两组120 min内胰岛素AUC比较差异无统计学意义。在等剂量亚麻籽油灌喂30 min后，HFD、FOD组血浆GLP-1水平升高，且FOD组GLP-1水平较HFD组高(P<0.01)。 结论 在高脂饮食的基础上长期补充亚麻籽油可改善小鼠的血糖水平及IR，其机制是通过促进肠道分泌GLP-1实现的，但需进一步研究证实。

　　【关键词】糖尿病;亚麻籽油;肥胖;胰升血糖素样肽-1

　　【Abstract】 Objective To explore the effects of flaxseed oil rich in n-3 fatty acid on plasma glucose level and GLP-1 secretion in C57BL/6J mice induced by high lipid dietary. Methods A[ total of 30 C57BL/6J mice were divided into three groups: normal dietary (ND) group, high fat dietary (HFD) group, and flaxseed oil dietary (FOD) group. After 8 weeks of treatment, intra-peritoneal glucose tolerance test (IPGTT), insulin tolerance test (ITT) and GLP-1 secretion were measured. Results Compared with HFD group, FPG and insulin were lower in FOD group (P<0.01). The IPGTT showed that the AUC of glucose in FOD group was lower than HFD group (P<0.01). The ITT demonstrated that the AUC of insulin was not significant different between FOD group and HFD group. After intragastric administration of the same dose of flaxseed oil 30 min in three groups, GLP-1 secretion were increased in FOD and HFD group, and FOD group was higher (P<0.01). Conclusion Long term supplement of flaxseed oil in C57BL/6J mice induced by high lipid dietary can improve the glucose level and IR. Further studies were needed to testify the possible underlying mechanism of stimulating the secretion of GLP-1.

　　【Key words】Diabetes mellitus;Flaxseed oil;Obesity;Glucagon-like peptide (GLP-1)