·糖尿病基础研究·

　　【摘要】 目的 探讨T2DM对OLETF大鼠不同器官组织胶原蛋白含量的影响。 方法 选取造模成功的T2DM OLETF大鼠(DM组)16只和同系LETO鼠(NC组)12只，分别采集皮肤、肝脏、股骨组织，进行HE、Masson染色，免疫组织化学法及高效液相色谱法测定胶原蛋白含量。 结果 与NC组相比，DM组皮肤、股骨组织胶原纤维减少，肝脏胶原纤维增多。DM组每克皮肤中胶原蛋白含量[(149.9±18.4) vs (266.0±54.8) mg/g]和股骨组织胶原含量[(185.60±19.38) vs (243.27±22.19) mg/g]均低于NC组(P<0.01或P<0.05);而肝脏胶原蛋白含量高于NC组[(134.54±18.78) vs (64.52±21.51) mg/g，P<0.01]。 结论 T2DM对不同组织胶原蛋白含量影响不同，皮肤、股骨胶原蛋白含量减少，肝脏胶原蛋白含量增加。

　　【关键词】 糖尿病，2型;皮肤;股骨;肝脏;胶原蛋白

　　· [Abstract] objective To research the effect of type 2 diabetes on collagen content in different organs in OLETF rats. Methods 24 male OLETF rats were given high-calorie diet for 30 weeks to set up the T2DM. At the 30th week, 16 male T2DM OLETF rats were selected as case group, and 12 LETO rats were used as control group. The skin, liver, and femur tissue were collected at the same time. HE and Masson staining and immunohistochemical test were performed to measure the content of collagen and HPLC was used to detect hydroxyproline. Results Compared with the NC group, DM group showed the collagen fibers were decreased in femoral and skin tissue, but increased in liver tissue. The collagen content of the skin tissue was significantly lower in DM group[ (149.9±18.4)mg/g] than in NC group[(266.0±54.8)mg/g] (P<0.01), and the collagen content of the femur tissue was also lower in DM group(185.60±19.38mg/g)than in NC group[(243.27±22.19)mg/g](P<0.05). In contrary, the collagen content of liver tissue was higher in DM group than in NC group[ (64.52±21.510 mg/g] (P<0.01). Conlusion The effect of type 2 diabetes on the collagen in different organs is different. The collagen content decreases in the femoral and skin tissue but increases in the liver.

　　[Key words] Diabetes mellitus，type 2; Skin; Femur; Liver; Collagen