来自:中国糖尿病杂志 编辑:梁同衍 王丽红 周生伟 杜红梅 李如江|点击数:|2015-04-09
·糖尿病基础研究·
【摘要】 目的 观察成年鼠糖尿病康复时胰岛β细胞特异基因的表达。 方法 建立自发康复糖尿病成年小鼠模型。在糖尿病自发康复过程中,以流式细胞术分选其胰岛β细胞,通过mRNA差异显示法研究胰岛β细胞基因差异表达,以免疫荧光双标显示其蛋白差异表达。 结果 在糖尿病自发康复过程中,以mRNA差异显示法在胰岛β细胞内共检出12个差异表达基因,其中雄激素受体(AR)在糖尿病最严重时低表达甚至不表达,在糖尿病康复过程中表达增强,在糖尿病完全康复时高表达。 结论 以mRNA差异显示法研究胰岛β细胞特异基因的表达切实可行。糖尿病康复时胰岛β细胞雄激素受体表达与功能性胰岛β细胞的增多密切相关。
【关键词】 糖尿病;胰岛β细胞;mRNA差异显示;雄激素受体
【Abstract】 Objective To observe the specific gene expressions of pancreatic β cells during spontaneous recovery in diabetic adult mice. Methods A diabetic adult mouse with spontaneous recovery was established. Pancreatic β cells of adult mice with recovery from diabetes were sorted by flow cytometry, then differential mRNA expressions of β cells were analyzed among groups via mRNA differential display, and their protein expressions were determined by double immunofluorescence. Results In the process of diabetes spontaneous recovery, 12 differential expression genes were obtained from pancreatic β cells by mRNA differential display, and AR expression in β cells was decreased significantly, even disappeared when the diabetes was the most serious. However, it gradually increased with recovery from diabetes, and was in a higher level when diabetes was recovered completely. Conclusion mRNA differential display is a powerful tool to study the differential gene expression in pancreatic βcells. The change of AR expression in β cells is strongly associated with an increase in the number of functional pancreatic β cells with recovery from diabetes.
【Key words】 Diabetes mellitus; Pancreatic β cell; mRNA differential display; Androgen receptor
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