·糖尿病基础研究·

　　【摘要】 目的 探讨血脂康对db/db糖尿病小鼠胰岛分泌功能和氧化应激(OS)的影响。 方法 选取db/db小鼠40只，随机分为血脂康组和db/db组，各20只，另选取db/m小鼠20只作为对照(NC)组，分别给予血脂康和安慰剂灌胃。每周监测代谢指标，8周后行腹腔葡萄糖耐量试验(IPGTT)，离体胰岛灌流评价胰岛分泌功能，免疫组织化学法分析胰岛内8-羟-2c-脱氧鸟苷(8-OHdG)，4-羟壬烯醛(4-HNE)和gp91phox的表达水平。 结果 干预8周后，血脂康组FPG低于为18.4mmol/L，db/db组小鼠的平均空腹血糖为25.9 mmol/L，两组比较有显著差异;血脂康组小鼠的平均空腹胰岛素水平位7.7mIU/L,显著低于db/db对照组(9.8 mIU/L)。免疫组化显示：与db/db组比较，血脂康组胰岛内8-OhdG，4- HNE和gp91phox的表达降低明显(P<0.05)。 结论 血脂康能降低胰岛OS反应，并改善葡萄糖代谢。

　　【关键词】 糖尿病;血脂康;胰岛分泌功能;氧化应激

　　【Abstract】 Objective To investigate the effects of Xuezhikang on pancreatic islet endocrine function and local oxidative stress in db/db diabetic mice. Methods A total of 40 male genetically diabetic C57BL/KsJ-db/db mice were randomly assigned into xuezhikang grouptreated with xuezhikang at 300 mg/(kg•d) and db/db group treated with placebo. In addition, another 20 age-matched lean non-diabetic C57BL/KsJ-db/m littermates served as normal control (NC) group. The metabolic indexes were measured every week. After 8 week, 10 mice were randomly selected from each group and received intraperitoneal glucose tolerance test (IPGTT). The in vitro insulin release kinetics was evaluated by perifusion system and the expression levels of gp91phox, 8-OhdG and 4-HNE were analyzed by immunohistochemical method. Results After 8 week treatment, FPG and FIns were lower in Xuezhikang group than in db/db group[FPG：(18.4±3.4)vs(25.9±2.2)mmol/L;FIns:(7.7±0.8)vs(9.8±1.4)mIU/L，P<0.05]. At the various time points after IPGTT, the area under curve of glucose was lower in Xuezhikang group than in db/db group[(1800.7±187.1)vs(2550.4±179.6), P<0.05], and at the 30 minute after IPGTT, the area under curve of insulin was higher in Xuezhikang groupthan in db/db group[(285.3±8.9)vs(268.5±10.4)，P<0.05]. Compared with the db/db group, since 1 minute point after high-concentration glucose stimulation, the insulin level increased remarkably in Xuezhikang group. Immunohistochemistry method showed that compared with db/db group, xuezhikang group showed the expression of gp91phox, 8-OhdG and 4-HNE was reduced (P<0.05). ConclusionXuezhikang can inhibit the local oxidative stress of pancreatic islet and improve glucose metabolism in db/db diabetic mice.

　　【Key words】 Diabetes mellitus; Xuezhikang; Islet endocrine function; Oxidative stress