[摘要] 目的   探讨沉默信息调节因子(SIRT1)对高糖诱导的系膜细胞内皮素-1（ET-1）和转化生长因子β1（TGF-β1）的关系。 方法  体外培养系膜细胞分为：(1)高糖组（高糖培养液）；（2）SIRT1激动剂（白藜芦醇）+高糖组，含1μmol/L白藜芦醇的高糖培养液；（3）SIRT1 RNAi干扰+高糖组，加入pTRC-shSIRT1慢病毒感染；（4）正常对照（NC）组。检测各组细胞SIRT1 基因表达，以及ET-1和TGF-β1水平。 结果高糖组、SIRT1激动剂+高糖组、SIRT1 RNAi干扰+高糖组及NC 组ET-1含量分别为（56.1±6.3）、（31.9±5.1）、（105.63±8.2）和（17.3±3.4）pg/ml；TGF-β1含量分别为（43.3±5.7）、（27.5±4.9）、（87.4±7.3）和（15.6±3.6）ng/ml。高糖抑制SIRT1表达，上调ET-1和TGF-β1表达（P＜0.05）；SIRT1激动剂可改善高糖对SIRT1的抑制，下调ET-1和TGF-β1表达（P＜0.05）；沉默SIRT1，可上调ET-1和TGF-β1表达（P＜0.05）。  结论  SIRT1基因对高糖诱导的系膜细胞ET-1和TGF-β1可能起负调控作用。

[关键词] 沉默信息调节因子1；内皮素-1；转化生长因子β1

Relationship of silent information regulator (SIRT1)withendothelin-1 and transforming growth factor β1 in mesangial cells induced by high glucose  

GU Wei，ZHANG Xue-kun，ZHAO Zheng-li，et al. Department of Endocrinology，Harrison International Peace Hospital，Hengshui 053000，China

【Abstract】Objective To investigate the relationship ofSIRT1 withendothelin-1 (ET-1) and transforming growth factor β1 (TGF-β1) in mesangial cells induced by high glucose.    Methods Mesangial cells(RMC) cultured in vitro were obtained and divided into fourgroups: ①high glucose group (cultured inhigh glucose medium); ②SIRT1 agonist (resveratrol) + high glucose group (cultured inhigh glucose medium with1μmol/Lresveratrol;③SIRT1 RNAi + high glucose group (transfected by pTRC-shSIRT1in high glucose medium); ④normal control group (NC group). Expression of SIRT1 gene weredetected. ET-1 and TGF-β1 were measuredby Elisa.Results ET-1leveland TGF-β1 level were56.1±6.3,31.9±5.1,105.63±8.2, 17.3±3.4pg/mland43.3±5.7,27.5±4.9,87.4±7.3, 15.6±3.6ng/mlin high glucose group, SIRT1 agonist (resveratrol) + high glucose group, SIRT1 RNAi + high glucose group and NC grouprespectively.Expression of SIRT1 gene was inhibited in high glucose environment.Expressions of ET-1 and TGF-β1were also up-regulatedin high glucose state (P < 0.05).The inhibition of high glucose on SIRT1 gene was improved and the expressions of ET-1 and TGF-β1 were down-regulatedby SIRT1 agonist(P < 0.05). The expressions of ET-1 and TGF-β1 were significantlyup-regulated by silencing SIRT1 gene (P < 0.05).     Conclusion    SIRT1 gene acts as a negative regulator forET-1 and TGF-β1 levels in mesangial cells induced by high glucose.

【Key words】Silent information regulator1; Endothelin-1; Transforming growth factor β1（TGF-β1)