【摘要】目的 探讨血管紧张素原(AGT)基因-20A/C多态性与DN关系。方法 应用聚合酶链反应一限制性片段长度多态性分析法(PCR-RFLP)对292例T2DM患者(正常白蛋白尿组148例，微量白蛋白尿组105例，大量白蛋白尿组39例) 及157名正常对照者(NC)进行AGT基因-20A/C多态性检测。结果 男性大量白蛋白尿组等位基因C频率显著高于正常白蛋白尿组(P=0.046)。Logistic回归分析显示,携带等位基因C(OR: 4.268,P=0.043，95%CI：1.044~17.447)与男性大量白蛋白尿相关。结论 AGT基因-20A/C多态性可能与浙江地区汉族人群男性T2DM患者出现大量白蛋白尿相关。

　　【关键词】糖尿病,2型 ;糖尿病性肾脏疾病;血管紧张素原基因;基因多态性

　　Study on relationship between -20A/C angiotensinogen gene polymorphisms and diabetic nephropathy of T2DM patients   MIAO Jing, YIN Xue-yao, YANG Jia-qi, et al. Department of Endocrinology, Sir Run Run Shaw Hospital, Hangzhou 310016, China

　　Corresponding author: LI Hong, E-mail: lihongheyi@126.com

　　【Abstract】Objective To investigate the relationship between angiotensinogen gene (AGT) -20A/C polymorphism and the susceptibility to type 2 diabetic nephropathy. Methods Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) to detect AGT gene -20A/C polymorphisms of 157 normal controls (NC) and 292 subjects with T2DM (148 in normo-albuminuria group, 105 in micro-albuminuria group, and 39 in macro-albuminuria group). Results In the male macro-albuminuria group, the frequency of C allele was significantly higher than in the normo-albuminuria group (P=0.046). The AGT gene -20A/C polymorphism (OR: 4.268, P=0.043, 95%CI: 1.044-17.447) was an independent risk factor for macro-albuminuria in male patients. Conclusion AGT gene -20A/C polymorphism might be a susceptibility gene for macro-albuminuria in male T2DM patients in Zhejiang Han population.

　　【Key words】 Angiotensinogen gene; Gene polymorphism; Diabetic nephropathy