·糖尿病基础研究·

　　【摘要】目的观察高脂喂养及吡咯列酮干预对SD大鼠视黄醇结合蛋白4(RBP4)水平的影响。方法雄性SD大鼠随机分为3组，分别给予普通饮食(NC组)、高脂(F组)及高脂吡咯列酮(F+Pio组)干预，第8、12周时行OGTT及胰岛素释放试验(ITT)，评价胰岛功能。检测FPG、血脂、肝功能及血清RBP4水平，并对肝脏、附睾脂肪和肾周脂肪称重，计算肝体比及脂体比。结果 8周后，F组FPG、血脂、胰岛素及OGTT血糖曲线下面积(AUCOGTT)、胰岛素曲线下面积(AUCITT)均高于NC组(P<0.05或P<0.01)，血清RBP4水平亦升高[(58.3±12.7)vs(26.8±7.3)，P<0.01];12周后，与F相比，F+Pio组FPG、胰岛素、TG、TC、AUCOGTT、AUCITT、脂体比、肝体比和血清RBP4水平降低[(30.3±13.9)vs(78.3±14.6)均降低(P<0.05或P<0.01)]。结论过氧化物酶体增殖物激活受体-γ激动剂激动剂吡咯列酮能减轻糖脂毒性、改善IR并降低体内RBP4水平。

　　【关键词】视黄醇结合蛋白4;胰岛素抵抗;腹内脂肪;过氧化物酶体增殖物激活受体-γ激动剂

The infulence of PPAR-γ agonists on serum RBP4 level in SD rats fed high-fat diet The infulence of PPAR-γ agonists on serum RBP4 level in SD rats fed high-fat diet  LIU Xiao-hua, WANG Ling-yan, ZHU Chao-yu,et al.Department of Endocrinology, The 6thPeople's Hospital Affiliated to Shanghai Jiaotong University，Shanghai 200233, China

Corresponding author: WEI li, E-mail：weili63@hotmail.com

    【Abstract】Objective To observe the impact of PPAR-γ agonist pioglitazone on serum RBP4 levels in SD rats fed high-fat diet. Methods Male SD rats were randomly divided into 3 groups: normal diet group, high fat diet group, high fat diet plus pioglitazone treatment group . At 8 and 12 weeks, oral glucose tolerance test (OGTT )and insulin-releasing test were performed to assay islet function. Also fasting blood glucose, lipid profiles, liver function, RBP4 levels were measured. Epididymal fat, perirenal fat, liver were weighed respectively for calculating fat to body weight ratio and liver to body weight ratio. Results After 8 weeks, fasting blood glucose, blood lipids, insulin, the area under curves (AUC) for glucose and for insulin were all higher in high fat diet group than in normal diet group. And serum RBP4 levels were also significantly higher in high fat diet groups than in normal diet group (58.3 ± 12.7 vs 26.8 ± 7.3, P<0.01). After 12 weeks, as compared with the high fat diet control group, high fat diet plus pioglitazone treatment group had lower levels of fasting blood glucose, insulin, triglycerides, total cholesterol levels, blood glucose area and insulin area under curve during OGTT as well as fat to body weight ratio, liver to body weight ratio (P<0.01 or P<0.05).Also, serum RBP4 levels were decreased significantly(30.3±13.9 ) vs(78.3±14.6)(P<0.01). Conclusion PPAR-γ agonist pioglitazone can significantly decrease serum RBP4 levels, meanwhile, significantly reduce the glucose and lipid toxicity, improve insulin resistance, and decrease RBP4 levels.

　　【Key Words】Retinol binding protein 4(RBP4);Insulin resistance(IR);Intra-abdominal fat;Peroxisome proliferator-activated receptor-γ(PPAR-γ)