·糖尿病分子生物学与遗传学·

　　刘大娜 郭敏 徐莹 姜晓艳

　　基金项目：黑龙江省科技攻关基金资助项目(GC05C40402)

　　作者单位：150001哈尔滨医科大学附属第一医院内分泌科(第一作者现工作于哈尔滨市第一医院)

　　通讯作者：姜晓艳，E-mail: jiangxy1963@126.com

　　【摘要】目的观察IL-6基因启动子区-174G/C及-572C/G多态性在中国汉族人群中的分布频率与T2DM患者大血管病变(DVC)的遗传易感性的关系。方法应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法检测480例患者(DM组120例，DVC组160例，对照组200例)-174位点、-572位点多态性。结果IL-6基因启动子区-174G/C多态性中，携带C等位基因的个体患DVC风险为G等位基因的1.8271倍(95%CI：1.1785~2.8327，P<0.01)。-572C/G多态性在DM+DVC组与对照组比较，差异无统计学意义(P>0.05)。结论IL-6-174G/C基因多态性与DVC的发病具有相关性。C等位基因可能是DVC的遗传易感基因。

　　【关键词】白细胞介素-6;基因多态性;糖尿病，2型;C反应蛋白;大血管合并症

　　doi:10.3969/j.issn.1006-6187.2012.04.006

　　The association between C-174G，G-572C polymorphisms of interleukin-6 (IL-6) gene and type 2 diabetic macrovascular diseasesLIU da-na, GUO min, XU ying，et al. Department of Endocrinology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China

　　Corresponding author: JIANG Xiao-yan，E-mail: jiangxy1963@126.com

　　【Abstract】ObjectiveTo study association between the polymorphisms in the promoter of interleukin-6 (IL-6) gene -174G/C and -572C/G macrovascular disease (MA) in type 2 diabetes mellitus (T2DM) in Chinese Han population.MethodsUsing the PCR-restriction fragment length polymorphism method, two IL-6 promoter polymorphism genes (-174G/C, -572C/G) were studied in 480 subjects including 120 diabetes without macrovascular disease,160 diabetes with macrovascular disease and 200 controls.ResultsFor -174G/C polymorphism in the promoter of IL-6 gene, the incidence of macrovascular disease in type 2 diabetes in the individuals with C allele was significantly increased in comparison with the carriers of G allele(OR=1.8271,95% CI：1.1785-2.8327，P<0.01). There was no statistical difference between DM+DVC and control groups for -572C/G polymorphism.ConclusionThese results suggest that the -174G/C polymorphism in the promoter of IL-6 gene is associated with macrovascular disease in type 2 diabetes. The C allele in -174G/C of IL-6 gene may contribute to the predisposition gene locus for macrovascular disease in type 2 diabetes.

　　【Key words】 Interleukin-6; Polymorphism(Genetics); Type 2 diabetes;C-reactive protein;Macrovascular disease