·糖尿病分子生物学与遗传学·

　　陈波 宋滇平 吴于滨 石柔 向茜

　　作者单位：650032昆明医学院第一附属医院糖尿病科(第一作者现在云南省第三人民医院肾内科)

　　通讯作者：宋滇平，E-mail：drsongdp@126.com

　　【摘要】目的探讨载脂蛋白C3(ApoC3)基因启动子区-482C→T多态性与中国人DN的相关性。方法运用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)，在中国人群中对179例T2DM患者和60名正常对照者(NC)的ApoC3基因启动子区-482C→T的多态性进行检测，并比较各组间基因型频率和等位基因频率以及相关临床资料。结果DN组(DN1+DN2)的T/T基因型频率及T等位基因频率高于DN0组(P<0.01)，但DN2与ND1组间T/T基因型频率及T等位基因频率差异无统计学意义(P>0.05)。T2DM组中， T/T基因型组患者的血TG水平及24h UAlb均高于C/T和C/C基因型组者(P<0.01)。Logistic回归分析表明：ApoC3基因启动子区-482C→T多态性和DM病程与DN显著相关，-482T/T基因型是DN发生的危险因素;DN患者中，DM病程>5年是DN2发生的危险因素。结论在中国南方汉族人群中，ApoC3基因启动子区-482T/T基因型可能是DN发生的危险因素;病程>5年可能是DN进展的危险因素。

　　【关键词】载脂蛋白C3基因;多态性;糖尿病肾病

　　doi:10.3969/j.issn.1006-6187.2012.04.007

　　The study of the association between ApoC3 gene promoter region -482C→T polymorphism and diabetic nephropathyCHEN Bo，SONG Dian-ping，WU Yu-bin,et al. Department of Diabetes,The First Affiliated Hospital of Kunming Medical College,Kunming 650032,China

　　Corresponding author: SONG Dian-ping，E-mail: drsongdp@126.com

　　【Abstract】ObjectiveTo investigate the association between ApoC3 gene promoter region insulin response element -482C→T polymorphism and diabetic nephropathy.MethodsThe polymorphism of ApoC3 gene promoter region -482C→T was detected by PCR-RFLP method in 179 unrelated Chinese with type 2 diabetes(DM group) and 60 normal controls (NC group). The genotype frequency, allele frequency and relative clinical data were compared between groups.RessultsThe frequencies of T/T genotype and T allele were significantly higher in DN (DN1+DN2) group than in DN0 group(P<0.01). However , there were no significant differences in genotype between DN1 and DN2 groups(P>0.05). In DM (DN0+DN1+DN2) group, triglyceride(TG) and 24h urinary albuminuria (UAlb) were significantly higher in T/T genotype group than in C/T and C/C genotype groups(P<0.01). Logistic regression analysis showed that ApoC3 gene promoter region-482C→T polymorphism and diabetic duration were risk factors for DN occurrence.Conclusion

　　The ApoC3 promoter region -482T/T genotype may be a risk factor of DN occurrence. Diabetic duration >5 years may be a risk factor for the progress of diabetic nephropathy in Chinese.

　　【Key words】ApoC3 gene; Polymorphism; Diabetic nephropathy