·糖尿病分子生物学与遗传学·

　　李会芳 吴于滨 苏真芳 王玉明 刘华 宋滇平

　　基金项目：国家自然科学基金资助项目(30760087,81160104);云南省自然科学基金面上项目(2004C0066M)

　　作者单位：650032昆明医学院第一附属医院糖尿病科(李会芳、吴于滨、苏真芳、宋滇平),检验科(王玉明、刘华)

　　通讯作者：宋滇平，E-mail:drsongdp@126.com

　　【摘要】目的探讨正常T细胞表达和分泌的活性调节蛋白(RANTES)基因启动子区-28C/G多态性与T2DM及其慢性并发症相关危险因素的相关性。方法运用PCR-RFLP技术对178例T2DM患者(T2DM组)和67例正常对照者(NC组)的RANTES基因-28C/G多态性进行检测。结果NC组和T2DM组间基因型和等位基因频率差异无统计学意义(P>0.05);RANTES基因-28G等位基因携带者血浆HDL-C浓度较非G等位基因携带者低且差异有统计学意义(P<0.05); Logistic回归分析： RANTES基因启动子区-28C/G多态性与HDL-C水平无关(P=0.228，OR=1.777)，BMI与HDL-C异常呈正相关(P=0.000，OR=2.562)。结论RANTES基因启动子区-28C/G多态性可能不是昆明地区汉族T2DM发生的危险因素，超重和肥胖可能是T2DM患者HDL-C 异常的危险因素。

　　【关键词】糖尿病;细胞周期蛋白类; 炎性

　　doi:10.3969/j.issn.1006-6187.2012.04.005

　　Study of the relationship between -28C/G polymorphism of RANTES gene promoter region and type 2 diabetes

　　LI Hui-fang，WU Yu-bin，SU Zhen-fang，et al. The Department of Diabetes，The First Affiliated Hospital of Kunming Medical College, Kunming 650032,China

　　Corresponding author: SONG Dian-ping, E-mail: drsongdp@we6.com

　　【Abstract】ObjectiveTo investigate the associatoin of RANTES (regulated on activation, normal T-cell expressed and secreted) gene promoter -28C/G polymorphism with type 2 diabetes mellitus (T2DM) and risk factors related to chronic complications.MethodsRANTES promoter -28C/G polymorphism was detected by PCR-RFLP in 178 T2DM patients and 67 normal controls (NC).ResultsThere were no statistical differences in genotype and allele frequencies between NC group and T2DM group. The level of HDL-C in patients with RANTES-28G-positive genotype was significantly lower than with -28G-negtive genotype (P<0.05).Logistic regression analysis showed RANTES promoter -28C/G polymorphism was not associated with the level of HDL-C. BMI was positivly correlated with HDL-C abnormality.ConclusionRANTES promoter -28C/G polymorphism may not be a risk factor for T2DM, while, overweight and obesity may be risk factors for HDL-C abnormality of patients with T2DM in Kunming Han people.

　　【Key words】Diabetes mellitus; Cyclins; Inflammation