·糖尿病基础研究·

　　郭兵 崔龙 刘瑞霞 肖瑛 石明隽 张国忠

　　基金项目：贵州省科技攻关项目(NO.2007-1033);贵州省高层次人才科研条件特助经费项目(NO.2007-44);贵州省科技基金项目(NO.2008-2167)

　　作者单位：550004贵阳医学院病理生理学教研室(郭兵、刘瑞霞、肖瑛、石明隽、张国忠);襄樊市中心医院肾内科(崔龙)

　　【摘要】目的研究Smad泛素化调节因子2(Smurf2)和核转录共抑制因子(SnoN)在T2DM大鼠肾组织中表达的动态变化及意义。方法建立T2DM模型,于成模8、12和16周处死。免疫组化和Western blot测肾组织Smurf2、SnoN、Tβ1和Smad2蛋白的表达;RT-PCR测肾皮质SnoN mRNA。结果DM大鼠肾组织Smurf2蛋白表达增多(P<0.01)，而SnoN蛋白的表达随病程进展逐渐减少(P<0.05)，两者呈显著负相关(r=-0.71, P<0.01); SnoN mRNA在各组肾组织中的表达差异无统计学意义(P>0.05)。结论T2DM肾病发病过程中，Smurf2蛋白的表达增加可能参与了SnoN蛋白的降解过程。

　　【关键词】Smad泛素化调节因子2;SnoN;糖尿病，2型

　　doi:10.3969/j.issn.1006-6187.2012.04.018

　　Changes of Smurf2 and SnoN expression in kidney tissues of type 2 diabetic rats and their biological rolesGUO Bing，CUI Long，LIU Rui-xia，et al. Department of Pathophysiology，Guiyang Medical College，Guiyang 550004，China

　　【Abstract】ObjectiveTo observe the changes of Smad ubiquitin regulatory factor 2 (Smurf2) and transcription co-repressor SnoN expression in renal tissues of type 2 diabetic rats and their biological roles.MethodsSD rats were injected with STZ after taking high fat and high sucrose diet for 12 weeks, and were killed thereafter at the 8th, 12th, and 16th weeks respectively. SnoN, Smurf2, TGF-β1, and Smad2 in renal cortex were detected by immunohistochemistry and Western blotting. SnoN mRNA was checked with RT-PCR.ResultsThe expression of Smurf2 in diabetic renal tissues was significantly increased (P<0.01), meanwhile the expression of SnoN was significantly decreased (P<0.05), and there was a negative correlation between these two proteins (r=-0.71, P<0.01). SnoN mRNA in diabetic renal tissues was unchanged among groups (P>0.05).ConclusionThe results suggest that the downregulation of SnoN expression may be mediated by Smurf2 in type 2 diabetic nephropathy.

　　【Key words】Smurf2; SnoN; Diabetes mellitus, type 2