【摘要】目的 探讨早老素相关菱形样蛋白(Presenilin associated rhomboid like Protein PARL)基因Leu262Val多态性与云南省临沧市沧源佤族人群糖尿病前期(PD)及糖尿病(DM)的相关性。方法 运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测263例PD、110例DM患者和127例健康对照者(NC)的PARL基因Leu262Val多态性。比较分析各组间基因型和等位基因频率以及相关临床资料，并进行问卷调查。结果 佤族PD组、DM组和NC组间的基因型和等位基因频率分布比较，各组中携带不同基因型个体的各项临床指标比较，差异均无统计学意义(P均>0.05)。女性、年龄、食用动物油可能是佤族人群PD和DM发生的危险因素。结论 在佤族人群中，PARL基因Leu262Val多态性可能与PD及DM 的发生无相关性; 改善生活方式可能防止PD和DM的发生。

　　【关健词】佤族;糖尿病前期;糖尿病;PARL 基因;多态性

　　Association of the Leu262Val polymorphism of PARL Gene and Pre-diabetes ,

　　Diabetes mellitus paitents in Wa People. DAI LI, LI Hui-fang , WANG Yu-ming , SONG Dian-ping , The 1st Affiliated Hospital of Kunming Medical College Kunming Yunnan 650032,China

　　【Abstract】Objective To explore the association of the Leu262Val polymorphism of PARL gene with Pre-diabetes and Diabetes mellitus paitents in Wa People. Methods The genetype was detected by PCR-RFLP in 263 Wa people with pre-diabetes (PD),110 Wa peopl with Diabetes mellitus(DM) and 127 healthy Wa people as normol controls (NC).The genotype and allele frequencies and relative clinic data were compared among groups, then recruit in the survey by questionnaire. Results There was no significant differences not only in genotypes and allele frequencies

　　distributions among the three groups , but also in the related clinical and biochemical indicators among the three groups with different genetypes (P>0.05).The female, age，edible animal oil were risk factors of PD and DM in Wa people. Conclusion The Leu262Val polymorphism of PARL may not be risk factors for PD and DM in Wa People. By improving the habits of life, it is hoped to prevent the development of PD and DM.

　　【Key words】Pre-diabetes; Diabetes mellitus;PARL gene; Polymorphism