·糖尿病分子生物学与遗传学·

　　项莹 谭宁 张众 李强 徐佳 顾明

　　基金项目：黑龙江省教育厅科学技术研究项目(10551175);黑龙江省卫生厅课题(2006-134);黑龙江省海外学人重点项目(1152hq34)

　　作者单位：150086 哈尔滨，哈尔滨医科大学附属第二医院内分泌代谢病科

　　通讯作者：李强，E-mail: hrblq@yahoo.com.cn

　　【摘要】 目的 探讨Ghrelin基因Leu72Met多态性与T2DM之间的相关性。 方法 采集316例T2DM患者和203名健康对照组(NGT组)人群的一般临床资料，利用PCR-RFLP进行Ghrelin基因Leu72Met的多态性分析。 结果 T2DM组的Leu72Met基因型分布及等位基因频率与NGT组相比差异无统计学意义。NGT Met72携带者血清LDL-C水平较同组Leu72纯合子个体增高(P<0.05)。 结论 Ghrelin基因Leu72Met分布与等位基因频率在T2DM和健康人群无明显差异，该基因多态性在健康人群可能与血清LDL-C水平相关。

　　【关键词】Ghrelin ;糖尿病，2型;基因多态性

　　doi:10.3969/j.issn.1006-6187.2011.10.003

　　Relationship between ghrelin gene Leu72Met polymorphism and type 2 diabetes mellitus

　　XIANG Ying, TAN Ning, ZHANG Zhong, et al. Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086,China

　　Correspording author: LI Qiang, E-mail: hrblq@yahoo.com.cn

　　【Abstract】 Objective To investigate the relationship between ghrelin Leu72Met polymorphisms and type 2 diabetes. Methods316 type 2 diabetic patients and 203 health people were genotyped for Leu72Met of ghrelin by PCR-RFLP assay. Anthropometric variables, blood glucose, insulin, lipids and other clinical parameters were measured. Results　In control group, the genotype frequencies were as follows: CC 88.2% (n=179), CA 10.3% (n=21), and AA 1.5% (n=3).In type 2 diabetes group, the genotype frequencies were as follows: CC 86.1% (n=272), CA 11.7% (n=37), and AA 2.2% (n=7).There were no significant differences in the genotype frequencies between control and T2DM groups. Serum LDL-C was higher in subjects carrying Met72+ genotype than in subjects with wild genotype in control group (P<0.05). Conclusion　The allele and genotype freguencies distribution of the Leu72Met is not different between control and type 2 diabetes groups; Leu72Met variants is related with serum LDL-C in control group.

　　【Key words】Ghrelin ; Diabetes mellitus，type 2; Gene; Polymorphism