·糖尿病分子生物学与遗传学·

　　孙保生任建功刘婧星罗晖胡雪剑张文博

　　作者单位：730030兰州大学第二医院糖尿病2科

　　通讯作者：任建功，E-mail:ren-jg@tom.com

　　【摘要】 目的 探讨甘肃地区PRKAA2基因rs2746342(G/T)单核苷酸多态性与T2DM及血清脂联素(APN)、抵抗素(Resistin)的关系。 方法 甘肃地区T2DM患者163例和正常对照组86名，两组年龄、性别匹配，采用聚合酶链反应/DNA限制性片段长度多态性(PCR/RFLP)、酶联免疫分析(ELISA)检测其基因多态性、空腹C肽(FC-P)、APN、Resistin，同时测身高、体重、血压、BMI、空腹血糖、血脂等。 结果 (1)T2DM组与正常对照组的基因型和等位基因频度分布存在显著差异(P<0.05);(2)T2DM组各基因型T/T、G/T、G/G 中Resistin、TG、TC、LDL-C、FC-P依次降低，APN、HDL-C依次增高(P<0.05);(3)APN水平在糖尿病超重组低于糖尿病正常体重组和对照组，Resistin水平在糖尿病超重组高于糖尿病正常体重组和对照组(P<0.05);(4)相关分析表明APN水平与BMI、血压负相关，与C-肽正相关(P<0.05)，Resistin水平与BMI、血压正相关，与C-肽负相关(P<0.05);(5)T2DM超重组较T2DM正常体重组和正常对照组TG、LDL-C水平升高(P<0.05)，HDL-C水平降低(P<0.05)。 结论 甘肃地区PRKAA2基因rs2746342(G/T)单核苷酸基因多态性可能与T2DM及胰岛素抵抗、血清APN、Resistin水平关联。

　　【关键词】糖尿病，2型;PRKAA2基因;单核苷酸多态性;脂联素;抵抗素

　　doi:10.3969/j.issn.1006-6187.2011.10.004

　　Association of the single nucleotide polymorphism (SNP) of the PRKAA2

　　with T2DM, adiponectin and resistin in Gansu, China

　　SUN Bao-sheng, REN Jian-gong, LIU Jing-xing, et al. The Second Department of Diabetes, The Second Hospital of Lanzhou University, Lanzhou 730030, China

　　Corresponding author: REN Jian-gong, E-mail:ren-jg@tom.com

　　【Abstract】 Objective To explore the relationship of polymorphism of AMPK rs2746342 gene with type 2 diabetes, serum adiponectin and resistin. Methods 163 type 2 diabetic patients and 86 normal controls were enrolled. PCR/RFLP was used to detect SNP. FC-P, serum adiponectin and resistin were detected by ELISA. Results(1)Ther were significant differences in genotype and allele freguency distribution between type 2 diabetes and normal control group(P<0.05);(2) From genotype T/T to G/T to G/G, the levels of resistin, TG, TC, LDL-C and F-CP were gradually decreased, and the levels of APN and HDL-C were gradually increased (all P<0.05).(3)APN level was lower in DM overweight group than in DM normal weight and non-DM group (all P<0.05).

　　Resistin level was higher in DM overweight than in DM normal weight and non-DM groups (all P<0.05). (4)There was a negative correlation between APN and BMI/BP, and a positive correlation between APN and fasting C-peptide (all P<0.05). Resistin was positively correlated to BMI and BP and negatively to fasting C-peptide (P<0.05). (5)In T2DM overweight versus normal weight and non-DM groups, the TG and LDL-C levels increased, and HDL-C levels decreased. Conclusions The polymorphism of PRKAA2-rs2746342 gene(G/T) may be associated with type 2 diabetes, insulin resistance, adiponectin and resistin.

　　【Key words】Diabetes,type 2 ; PRKAA2 gene; Single nucleotide polymorphism; Adiponectin; Resistin