糖尿病大血管并发症

　　【摘要】 目的 研究T2DM合并大血管病变患者血清松弛素(RLX)、基质金属蛋白酶-2(MMP-2)的表达水平，探讨RLX与MMP-2的关系。 方法 将80例T2DM患者根据是否合并大血管病变分为T2DM未合并大血管病变(T2DM)组40例和T2DM合并大血管病变(MCV)组40例，另选体检健康者40名作为正常对照(NC)组。采用ELISA检测各组血清RLX、MMP-2水平。 结果 NC、T2DM和MCV 组血清RLX水平逐渐下降[(2211.35±217.66) vs (1600.00±133.18) vs (1349.43±173.98)pg/ml，P<0.01]，MMP-2水平逐渐升高[(9.00±1.00) vs (9.66±1.23) vs (12.54±1.25)μg/L，P<0.05或P<0.01)。Pearson相关性分析结果显示，RLX与SBP、DBP、FPG、HbA1c、LDL-C和MMP-2呈负相关，MMP-2与病程、BMI、SBP、DBP、FPG、HbA1c、TG和LDL-C呈正相关，与HDL-C、RLX呈负相关。多元线性回归分析结果显示，HbA1c、MMP-2是RLX的独立危险因素，SBP、LDL-C和RLX是MMP-2的独立危险因素。 结论 T2DM合并大血管病变患者血清RLX水平下降，MMP-2水平升高，血清RLX、MMP-2水平与T2DM大血管病变相关。

　　【关键词】糖尿病，2型;大血管病变;松弛素;基质金属蛋白酶-2

　　【Abstract】 Objective To investigate the levels of serum relaxin (RLX) and matrix metalloproteinase-2 (MMP-2) in T2DM patients with macroangiopathy, and evaluate the relationship between RLX and MMP-2. Methods A total of 80 T2DM patients were divided into two groups: without macrovascular complications (T2DM) group (n=40), with macrovascular complications (MCV) group (n=40). Healthy subjects were enrolled as normal control (NC) group (n=40). Serum RLX and MMP-2 levels were measured by ELISA in three groups. Results In NC, T2DM and MCV group, RLX levels were decreased significantly [(2211.35±217.66) vs (1600.00±133.18) vs (1349.43±173.98)pg/ml，P<0.01], MMP-2 levels were increased significantly [(9.00±1.00) vs (9.66±1.23) vs (12.54±1.25)μg/L，P<0.05 or P<0.01). Pearson correlation analysis showed that RLX levels were negatively correlated with SBP, DBP, FPG, HbA1c, LDL-C and MMP-2. MMP-2 level were positively correlated with duration, BMI, SBP, DBP, FPG, HbA1c, TG and LDL-C, and were negatively correlated with HDL-C and RLX. Multiple stepwise regression analysis showed that HbA1c and MMP-2 were the independent related factors for RLX levels. SBP, LDL-C and RLX were the independent related factors for MMP-2 levels. Conclusion In T2DM patients with macroangiopathy, RLX levels were decreased, but MMP-2 levels were increased. Serum RLX and MMP-2 were associated with macroangiopathy in T2DM.

　　【Key words】Diabetes mellitus，type 2;Macroangiopathy;Relaxin (RLX);Matrix metalloproteinase-2 (MMP-2)