【摘要】 目的   探讨T1DM患者血浆miR-126表达水平的变化及其临床意义. 方法   选取T1DM患者36例及健康对照(NGT)者34名，RT-PCR检测各组血浆miR-126，并分析其与年龄、BMI、高敏C反应蛋白(hsC-RP)、内皮型一氧化氮合酶(eNOS)、FPG、2 hPG、HbA^₁C、FC-P、2 hC-P、HDL-C、LDL-C、TG、TC的关系。 结果   与NGT组比较，TIDM组血浆miR-126表达下降[(1.65±0.61)VS
(0.68±0.18)，P<0.053。相关分析显示，miR-126与HDL-C、FC-P、2 hC-P及eNOS呈正相关(P<0.05或P<0.01)，与LDL-C、TC、hsC-RP及年龄呈负相关(P<0.05或P<0.01)；多元逐步回归分析显示，hsC-RP、FC-P、TC、年龄是miR-126的影响因素. 结论   TIDM患者血浆miR-126呈低表达，可能与胰岛功能及内皮功能有关，hsC-RP及TC是miR-126的独立影响因素，miR-126可能参与了TIDM患者的炎症反应及脂质代谢异常，且miR-126水平随年龄增长而下降。
    【关键词】糖尿病，1型；miR-126；炎症反应；糖脂代谢；胰岛功能
    【Abstract】 Objective   To investigate the changes of plasma miR-126 expression leveI in patientswith type 1 diabetes(T1DM). Methods   The plasma miR-126 expression level was investigated in 36T1DM patients and 34 healthy controls (NGT)by real-time fluorescent quantitative polymerase chainreaction(PCR)．The relationship between plasma miR-126 and clinical indicators such as age，BMI，hsC-RP，eNOS，FPG，2 hPG，HbA1c, FC-P, 2 hC-P，HDL-C，LDL-C，TG and TC was analyzed by correlation
analysis．The main influencing factors for miR-126 were analyzed by multiple stepwise regression analysis．Results   The miR-126 expression level was significantly decreased in T1DM group than that in NGT group[(1.65±0.61)VS(0.68±0.18)，P<0.05]．Correlation analysis showed that miR-126 was positivelycorrelated with HDL-C，FCP，2 hC-P and eNOS(P<0.05 or P<0.01)，and negatively correlated with LDL-C，TC，hsC-RP and age(P<0.05 or P<0.01)．Multiple stepwise regression analysis showed that hsC-RP，FC-P，TC and age were independent influencing factors for miR-126.    Conclusion    The expression level of plasma miR-126 is lower in TIDM group than in NC group，which is possibly associated with islet and endothelial functions．hsC-RP and TC are independent influencing factors for miR-126，which may be involved in the inflammatory reaction and 1ipid metabolism disorder in T1DM patients．With age，the expression level of miR-1 26 may decrease．
    【Key words】  Diabetes mellitus, type 1；miR-126；Inflammatory reaction；Glucolipid metabolism；
Islet function