【摘要】 目的研究不同运动量对糖尿病动脉粥样硬化大鼠主动脉组织Toll样受体2(TLR2)、TLR4及白细胞介素6(IL-6)、核转录因子IcB(NF-_KB)表达的影响。 方法将100只SD大鼠随机分为对照组(n=20)和糖尿病动脉粥样硬化组(n=80)。对照组予普通饲料喂养，糖尿病动脉粥样硬化组予高糖高脂喂养后腹腔注射STZ(30 mg／kg)建立糖尿病动脉粥样硬化模型，后再随机分为模型亚组及小、中、大运动量亚组，对后3亚组进行游泳运动干预。干预后采用ELISA测定各组血浆IL-6、NF-_kB的浓度及采用Western blott测定主动脉组织中TLR2、TLR4的表达。 结果   运动前，模型亚组血浆IL -6[(58．06±5．38)到s(14.21±

4.04)ng／m1]、NF-_KB(154.24±9.24)'US(65．99±8．63) ng／m1]较对照组升高(P<0.01)；各运动亚组运动干预后血浆IL-6、NF-IcB较运动前下降(P<0.01)。模型亚组主动脉组织中TLR2E(1.19±0．04)V5(0.57±0.04)]、TLR4蛋白表达[(1.25±0.05)VS(0.68±0.06)]较对照组升高(P<0.01)；运动干预的各亚组运动后主动脉组织中TLR2、TLR4较模型亚组降低以中、大运动量亚组下降明显(P<0.01或P％0.05)。 结论糖尿病动脉粥样硬化大鼠动脉组织TLR2、TLR4蛋白表
达及血浆IL-6、NF-xB增高，运动可以改善上述与促炎通路有关的因素。

    【关键词】糖尿病，2型；动脉粥样硬化；运动；Toll样受体；炎症因子
    【Abstract】 Objective  To study the effect of different volume of swimming exercise on the expressions of toll-like receptor2(TLR2)and TLR4 in aortic tissue，and the concentrations of IL，-6 andNF-_KB in plasma in rats with diabetic atherosclerosis． Methods   100 Sprague-Dawley rats were randomlyassigned into control group(n=20)and diabetic atherosclerosis group(n=80)．The rats in control group were fed with regular diet．Diabetes was induced by feeding with high-fat-high-sugar diet and intraperitoneal streptozotocin injection(30 mg/kg)．The diabetic atherosclerosis group was divided intofour subgroups：the model subgroup，the low volume exercise subgroup，the moderate volume exercisesubgroup and the high V01Ume exercise subgroup．The swimming time was determined 30 minutes for thehigh volume exercise subgroup，2 1 minutes for the moderate volume exercise subgroup，1 2 minutes for thelow volume exercise subgroup in this study．The concentrations of IL-6 and NF-_KB were tested by ELISA．The expressions of TLR2 and TLR4 in aorta tissue were tested by Western-Blotting． Results   The concentrations of IL-6 and NF-IcB in plasma in the model subgroup were higher than those in control group[(58.06±5．38)VS(14.21±4.04)ng/ml，and(154．24+

9.24)VS(65.99±8.63)ng／ml，respectively](P<0.01)．The concentrations of IL，-6 and NF-KB were decreased after exercise in each intervention group. The expressions Of TLR2 and TLR4 in aortic tissues in model subgroup was higher than those in control group [(1.19±t-0．04)狮(0.57±0.04)，and(1.25±0.05)vs(0.68±0.06)，respectively](P<0.01)．Exercise decreased the expressions of TLR2 and TLR4 in aortic tissues especially in the moderatevolume exercise and the high volume exercise subgroups． Conclusion The concentrations of plasma IL-6 and NF-KB and the expressions of TLR2 and TLR4 in aortic tissue were increased in diabetic atherosclerosis rats．Exercise can improve、the factors related to pro-inflammatory pathways．
  【Key words】  Diabetes mellitus，type 2；Atherosclerosis；Exercise；TLRs；Inflammatory factors