【摘要】 目的探讨TN卜q、IL-6基因对高血糖“代谢记忆”的调控作用。 方法 根据正常对照(NC)组、糖尿病(DM)组、代谢记忆组对90只雄性健康SD大鼠进行造模，各30只。RT-PCR测定mR—NA表达；TNF-a、IL-6的RT-PCR产物电泳条带积分光密度值(ROD)；TNF-a、IL-6指标在血清中的表达情况。 结果与NC组比较，代谢记忆组TNF-a、IL-6的RT-PCR产物ROD呈降低趋势，DM组呈升高趋势(P<0.05)；与NC组比较，代谢记忆组血清中TNF-a、IL-6水平呈降低趋势(P<0.05)，DM组呈升高趋势(P<0.05)。 结论NC组TNF-a和IL-6的mRNA低于DM组，代谢记忆组(高血糖16～32 mmol／L 12周在先，正常血糖5～

8 rnmol／L 12周在后)的相应mRNA低于NC组。同上的规律在血清 腓a和IL-6水平再次出现。这是临床糖尿病代谢记忆的分子生物学基础在T卜Ba和IL-6的表现。
     【关键词】 高血糖；代谢记忆；TNF-a；IL-6；基因表达；调控

【[Abstract】 Objective  To explore regulation of TNF一旺and IL-6 genes On‘metabolic memory'of
hyperglycemia． Methods   A total of 90 healthy male SD rats were divided into three groups as control
group(NC group，n=30)，diabetes mellitus group(DM group，n=30)，metabolic memory group(n=30)．
mRNA expression was measured by RT-PCR．Electrophoretic bands from TNF-a and IL-6 RT-PCR
products were observed for integral optical density(ROD)．Serum 1evel of TNFa and IL-6 were also
tested． Results   ROD of TNF-a and IL-6’S RT-PCR products were decreased in metabolic memory
group，increased in DM group as compared with NC group(P<0.05)．Serum level of TNF-a and IL，6
were decreased in metabolic memory group，increased in DM group as compared with NC group(P<
0.05)． Conclusion   mRNA expression and serum level of TNF-n and IL-6 iS 10wer in NC group than in
DM group，and also lower in metabolic memory group than in NC group．This finding illustrates the
molecular biological mechanism of metabolic memory of diabetes mellitus by TNF-一“and IL-6．
【Key words】 High blood glucose；Metabolic memory；TNF-a；IL-6；Gene expression；Regulation