糖尿病临床研究

　　【摘要】 目的 探讨LADA与溃疡性结肠炎(UC)患者外周血T细胞亚群变化及淋巴细胞失衡的临床意义。 方法 选取LADA、UC组各52例和健康对照(NC)组52名，采用单克隆抗体间接免疫荧光技术检测各组外周血淋巴细胞CD3+、CD4+、CD8+亚群百分比。 结果 LADA、UC、NC组CD3+亚群比较差异均无统计学意义[(62.29±4.97)% vs (63.49±5.12)% vs (61.37±4.83)%，P>0.05]。UC组CD4+亚群较LADA、NC组降低[(27.63±6.85)% vs (35.87±5.92)%，P<0.01;(27.63±6.85)% vs (34.63±5.39)%，P<0.01]。LADA组CD8+亚群较UC、NC组升高[(27.54±3.05)% vs (22.21±3.46)%，P<0.01;(27.54±3.05)% vs (21.82±3.77)%，P<0.01]。LADA、UC组CD4+/CD8+较NC组降低[(1.35±0.37) vs (1.78±0.44)，P<0.01;(1.32±0.51) vs (1.78±0.44)，P<0.01]。 结论 LADA、UC患者均出现淋巴细胞CD4+、CD8+ 亚群失衡，淋巴细胞亚群失衡参与两者发病，但具体机制不同。

　　【关键词】成人隐匿性自身免疫性糖尿病;溃疡性结肠炎;T细胞亚群失衡

　　【Abstract】Objective To detect changes of peripheral blood T cell subsets in patients of LADA and ulcerative colitis (UC) in order to investigat the clinical significance of T cell subsets imbalance. Methods 52 cases of LADA, 52 cases of UC and 52 normal control (NC) people were selected，and peripheral blood T lymphocyte CD3+, CD4+, CD8+ subsets have been quantified by an indirect immunofluorescence technique using monoclonal antibodies. Results There were no statistically difference in the percentage of CD3+ subsets among three groups [(62.29±4.97)% vs (63.49±5.12)% vs (61.37±4.83)%，P>0.05]. The percentage of CD4+ subsets was lower in UC group than in LADA and NC group[(27.63±6.85)% vs (35.87±5.92)%，P<0.01;(27.63±6.85)% vs (34.63±5.39)%，P<0.01]. The percentage of CD8+ subsets was higher in LADA group than in UC and NC group [(27.54±3.05)% vs (22.21±3.46)%，P<0.01;(27.54±3.05)% vs (21.82±3.77)%，P<0.01]. The ratio of CD4+/CD8+ were higher in LADA group and UC group than in NC group [(1.35±0.37) vs (1.78±0.44)，P<0.01;(1.32±0.51) vs (1.78±0.44)，P<0.01]. Conclusion The CD4+/CD8+ lymphocytes imbalance were detected in LADA and UC. The results suggested that lymphocyte subsets imbalance are involved in the the incidence of both diseases，but the extensional pathogenesis was incomplete the same.

　　【Key words】Latent autoimmune diabetes in adult (LADA);Ulcerative colitis (UC);T cell subsets imbalance