【摘要】 目的 观察基质细胞衍生因子-1(SDF-1)对糖尿病患者外周血内皮祖细胞(EPCs)功能的影响。 方法 选择糖尿病患者(DM组)20例和健康者(NC)10名，获取外周血单个核细胞，培养4 d后鉴定EPCs。分别将两组随机分为NC1、NC2、DM1和DM2亚组。NC1、DM1亚组采用SDF-1进行干预，第7 d检测EPCs迁移能力和血管形成能力。 结果 (1)DM2亚组EPCs迁移能力和血管形成能力均低于NC2亚组[(15.500±2.224) vs (21.200±1.304)个,(113.870±15.198) vs (181.800±9.202) μm，P<0.001]。与NC2亚组比较，NC1亚组EPCs迁移能力和血管形成能力均增强[(24.60±1.517) vs (21.20±1.304)个，(197.98±10.855) vs (181.80±9.202) μm，P<0.05];与DM2亚组比较，DM1亚组EPCs迁移能力和血管形成能力增强[(19.300±1.337) vs (15.500±2.224)个，(140.520±10.622) vs (113.870±15.198) μm，P<0.001];(2)加入SDF-1干预后，DM组EPCs迁移能力和血管形成能力增强的幅度均高于NC组，比较差异有统计学意义(P=0.036，0.006);(3)SDF-1与EPCs迁移能力及血管形成能力呈正相关(r=0.488、0.428，P<0.01、P<0.05)。 结论 SDF-1可增强外周血EPCs迁移能力和血管形成能力，对于糖尿病患者效果更为明显。

　　【关键词】 基质细胞衍生因子-1;内皮祖细胞;糖尿病

　　【Abstract】 Objective To observe the influence of stromal cell-derived factor-1 (SDF-1) on peripheral endothelial progenitor cells’(EPCs) in patients with diabetes. Methods Collect 20 diabetes patients (DM group) and 10 healthy people (NC group) were collected in this study. Peripheral blood mononuclear cell was obtained to identify the EPCs after four days. DM group and NC group were divided into NC1,NC2,DM1 and DM2 subgroup. NC1 and DM1 subgroup were cultivated by 100 ng/ml SDF-1 ,and tested EPCs’ migration and angiogenesis ability after seven days. Results (1) EPCs’migration and angiogenesis ability was lower in DM2 subgroup than in group NC2 subgroup [(15.500±2.224) vs (21.200±1.304) cases,(113.870±15.198) vs (181.800±9.202) μm，P<0.001]. EPCs’migration ability and angiogenesis ability in NC1 subgroup was higher than in NC2 subgroup [(24.60±1.517) vs (21.20±1.304) cases，(197.98±10.855) vs (181.80±9.202) μm，P<0.05]; EPCs’migration ability and angiogenesis ability was higher in DM1 subgroup than in DM2 subgroup[(19.300±1.337) vs (15.500±2.224) cases，(140.520±10.622) vs (113.870±15.198) μm，P<0.001]. (2) After add SDF-1, the extent of enhancement of EPCs’ migration and angiogenesis ability were higher in DM group than in HC group(P=0.036，P=0.006). (3)There was a positive correlation of SDF-1 with EPCs’ migration and angiogenesis ability(r=0.488、0.428，P<0.01、P<0.05). Conclusions SDF-1 can increase the chemotactic migration and angiogenesis ability of the EPCs from peripheral blood, especially for the diabetes.

　　【Key Words】 Stromal cell-derived factor-1;Endothelial progenitor cells(EPCs); Diabetes mellitus