[摘要] 目的 比较T2DM患者、牙周病患者与健康者龈沟液中基质金属蛋白酶-1(MMP-1)和龈沟液(GCF)量，以及T2DM患者龈沟液中MMP-1量与糖代谢状况的关系。方法 使用滤纸条法采集龈沟液。选取T2DM组、T2DM合并牙周病组30例，健康对照组30名，ELISA测定龈沟液中MMP-1含量，运用高效液相色谱法HbA1c水平。结果 糖尿病组和T2DM合并牙周病组龈沟液的量高于正常对照组[(2.81±1.58)、(3.56±2.96) vs (1.02±0.65) μl/样本](P<0.01)。T2DM合并牙周病组GCF中MMP-1量高于糖尿病组和正常对照组[(5.02±0.16) vs(3.01±0.09)、(2.24±0.11) μl/样本](P<0.01)。糖尿病组与正常对照组GCF中MMP-1量差异无统计学意义(P>0.01)。T2DM合并牙周病组GCF中MMP-1量与HbA1c水平无相关性(r=0.153,P=0.151)。 结论 3组GCF及MMP-1水平在T2DM合并牙周病组中最高，在正常对照组最低，提示牙周炎症是影响结果的主要原因。MMP-1水平在糖尿病组中低于T2DM合并牙周病组，提示糖尿病患者不只是MMP-1水平改变而导致牙周病的易感，可能是多个因素共同作用的结果。

　　[关键词]糖尿病，2型;牙周病;龈沟液;基质金属蛋白酶-1

　　[Abstract] Objective To compare the MMP-1 and gingival crevicular fluid (GCF) levels in GCF of patients with type 2 diabetes mellitus, periodontal disease, and healthy persons, and to investigate the relationship between MMP-1 concentration in GCF of T2DM patients and glycometabolic condition. Methods GCF collection was performed with the filter paper method. Thirty patients of T2DM group and T2DM merging periodontal diseases group, and thirty healthy controls were selected in this study. The MMP-1 levels in GCF were measured by ELISA, and HbA1c levels were measured by high-performance liquid chromatography. Results The diabetic group and T2DM group with periodontal diseases had higher GCF as compared to the normal control group (P<0.01). The MMP-1 level in GCF of T2DM group with periodontal diseases was higher than that in the diabetic group and the normal control group [(5.02±0.16) vs (3.01±0.09), (2.24±0.11) μl/sample] (P<0.01). The MMP-1 level in GCF of diabetic group and normal control group had no significant difference (P>0.01). The MMP-1 level in GCF of T2DM group with periodontal diseases was not correlated with HbA1c levels. Conclusion Among three groups, GCF and MMP-1 levels are the highest in T2DM group with periodontal diseases and the lowest in normal control group, which suggested that periodontitis is the main reason affecting the results. MMP-1 level is lower in diabetic group than that in T2DM group with periodontal diseases, which suggested that the periodontal disease susceptibility in diabetics is not just caused by changes of MMP-1 level, but might be the results of several factors working together.

　　[Key words] Diabetes mellitus, type 2; Periodontal disease; Gingival crevicular fluid; Matrix metalloproteinases-1