·糖尿病基础研究·

　　【摘要】 目的  观察糖尿病动脉粥样硬化(AS)大鼠血清中二羰基化合物的表达。方法：20只2型糖尿病Goto-Kakizaki (GK)大鼠采用左旋硝基精氨酸甲酯(L-NAME)和高脂饲料喂养法制备糖尿病动脉粥样硬化大鼠模型，另选10只Wistar大鼠作为对照组。应用气相色谱-质谱联用仪(GC-MS)对大鼠血清进行分离、纯化，测定其中二羰基化合物甲基乙二醛(methylglyoxal，MG)和3-脱氧葡萄糖醛酮(3-Deoxyglucosone，3-DG)的含量。结果：糖尿病动脉粥样硬化模型组大鼠血清中二羰基化合物MG和3-DG含量分别为216.7±15.6μmol/L和19.5±3.8μmol/L，与对照组(85.3±24.9μmol/L和8.2±2.4μmol/L)相比，含量明显升高(P<0.05)。结论：二羰基化合物含量升高可能是糖尿病动脉粥样硬化的发病机制之一。

　　【关键词】动脉粥样硬化;糖尿病;乙二醛;脱氧葡萄糖;气相色谱-质谱法

Detection of the concentration of dicarbonyl compounds in serum of diabetic atherosclerosis rats CHEN Yu-jing, HUANG Xiao-bo, CHEN Wen-qiang, et al. Xuanwu Hospital, Capital Medical University, Beijing100053, China

　　【Abstract】 Objective To detect the concentration of dicarbonyl compounds in serum of diabetic atherosclerosis rats. Methods Twenty Goto-Kakizaki (GK) rats were randomized into the control and model groups. The diabetic atherosclerosis rat models were produced by feeding with NG-Nitro-L-arginine methyl ester (L-NAME) and high fat and sucrose diet. Another 10 Wistar rats were taken as the controls. Gas chromatography-mass spectrometry (GC-MS) was applied to determine the concentration of dicarbonyl compounds methylglyoxal (MG) and 3-Deoxyglucosone (3-DG) in serum.

　　Results The concentration of MG and 3-DG in diabetic atherosclerosis rats (216.7±15.6μmol/L and 19.5±3.8μmol/L respectively) was significantly higher than in the control group (85.3±24.9μmol/L and 8.2±2.4μmol/L respectively) (P<0.05). Conclusion The elevated levels of dicarbonyl compounds may be one of the mechanisms of diabetic atherosclerosis.

　　【Key Words】 Atherosclerosis; Diabetes mellitus; Glyoxal; Deoxyglucose; Diabetic atherosclerosis; Gas chromatography-mass spectrometry (GC-MS)