·糖尿病基础研究·

　　【摘要】　目的 探讨枸杞多糖对T2DM大鼠周围神经病变的保护作用及其机制。 方法 SD大鼠制备成T2DM大鼠模型后，随机分为模型组、枸杞多糖小剂量组、大剂量组及α-硫辛酸对照组。治疗10周后检测各组大鼠坐骨神经的传导速度，并测该组织中MDA及GSH-Px含量，观察其超微结构。 结果 与模型组相比，枸杞多糖大剂量组能改善神经传导速度[(32.31±3.54) m/s vs(27.11±5.99)m/s，P<0.05)]，降低坐骨神经组织中MDA水平[(1.80±1.03) nmol/ml vs(2.32±1.02)nmol/ml，P<0.05)]提高GSH-Px水平[(107.44±16.03 )U/gprot vs (67.83±28.91 U/gprot， P<0.05)]。模型组大鼠坐骨神经超微结构有明显的损伤反应，经枸杞多糖干预后病变程度减轻。 结论 枸杞多糖通过抗氧化应激，可以提高神经传导速度，并同时改善周围神经的结构和功能，具有保护糖尿病周围神经病变的作用。

　　【关键词】 枸杞多糖; 糖尿病，2型;大鼠 ;周围神经病变

Protective effect of Lycium Barbarum Polysaccharides on type 2 Diabetic Rats with peripheral neuropathy WU Qing-qiu, HE Lan-jie. The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China

Corresponding author: HE Lan-jie, E-mail: helanjie@medmail.com.cn

　　【Abstact】 Objective To explore the protective effect of Lycium Barbarum Polysaccharides (LBP) on type 2 diabetic rats with peripheral neuropathy and its mechanism. Methods SD rats were induced hyperglycemia and randomly divided into the model group, LBP low- and high-dose groups, and α-Lipoic acid group. The course of treatment lasted for 10 weeks. The conduction velocity of sciatic nerve of all groups was detected and the contents of MDA and GSH-Px in sciatic nerve were determined, and the changes in its ultrastructure was observed. Results Compared with the model group, the LBP high dose group could accelerate the nerve conduction velocity [(32.31±3.54) m/s vs (27.11±5.99) m/s, (P<0.05)], attenuate the content of MDA in sciatic nerve [(1.80±1.03) nmol/ml vs (2.32±1.02) nmol/ml, (P<0.05)], and elevate the content of GSH-Px [(107.44±16.03) U/gprot vs (67.83±28.91) U/gprot, (P<0.05)]. There were obvious reactions in the ultrastructure of sciatic nerve in the model group. However, the pathological condition in sciatic nerve was alleviated after the intervention with LBP. Conclusion LBP could accelerate the nerve conduction velocity through anti-oxidative stress, at the same time, it could ameliorate the structure and function of peripheral nerves, thus protecting the type 2diabetic rats with peripheral neuropathy.

　　【Key words】Lycium Barbarum Polysaccharides (LBP); Diabetic type 2; Rats; Peripheral neuropathy