·糖尿病基础研究·

　　【摘要】 目的 研究NLRP3炎症复合体在高糖培养的大鼠近端肾小管上皮细胞(NRK-52E)中的表达及其与细胞凋亡的关系。方法 将NRK-52E细胞分为正常组、等高对照组、高糖组、高糖+阴性对照组及高糖+干扰组。培养48 h后应用western blot检测各组NLRP3、凋亡相关斑点样蛋白(ASC)及天冬氨酸蛋白水解酶1、3(caspase-1、3)的表达情况。细胞凋亡情况采用流式细胞仪检测。结果 高糖刺激可上调NRK-52E细胞NLRP3炎症复合体的表达及Caspase-3的表达(P均<0.05)，细胞凋亡率升高(P<0.05);通过特异性的SiRNA抑制NLRP3蛋白质的表达后，细胞NLRP3炎症复合体及Caspase-3的表达均减少(P均<0.05)，细胞凋亡率下降(P<0.05)。结论 高糖促进NRK-52E细胞NLRP3炎症复合体表达，针对NLRP3的SiRNA通过减少NLRP3炎症复合体的表达从而抑制高糖诱导的细胞凋亡。

　　【关键词】 糖尿病慢性肾脏疾病;葡萄糖;NLRP3炎症复合体;RNA干扰;凋亡;大鼠

The expression of NLRP3 inflammasome in high glucose cultured renal tubular epithelial cells and its relationship with apoptosis WANG Wei, LI Rong-shan, ZHAO Hong-yu, et al. Department of Nephrology, The Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China

Corresponding author: LI Rong-shan, Email: rongshanli@yahoo.com.cn

　　【Abstract】 Objective To study the expression of NLRP3 inflammasome in high glucose cultured renal tubular epithelial cells (NRK-52E cells) and its relationship with apoptosis. Methods NRK-52E cells were cultured in high glucose and randomized into 5 groups: control group, isotonic control group, high glucose group, high glucose + negative control group, and high glucose + interference group. After 48 hours, the expressions of NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), and Caspase-1 and 3 were detected by Western blotting. The apoptotic rate of NRK-52E cells was measured by flow cytometry. Results High glucose could increase the expressions of NLRP3 inflammasome of NRK-52E cells and caspase-3 (P<0.05), and the apoptotic rate was increased (P<0.05). Through the specific inhibition of SiRNA NLRP3 expression, the levels of cellular NLRP3 inflammasome and Caspase-3 decreased in the high glucose + interference group (P<0.05), and the apoptotic rate decreased significantly (P<0.05). Conclusion High glucose increases the expression of NLRP3 inflammasome of NRK-52E cells. It is through reducing the expression of SiRNA NLRP3 inflammasome to inhibit high glucose-induced apoptosis.

　　【Key words】Chronic kidney disease in diabetes; Glucose, NLRP3 inflammasome, RNA interfering; Apoptosis; Rats