·糖尿病基础研究·

　　脂联素球状结构域对2型糖尿病大鼠己糖激酶和丙酮酸激酶活力的影响

　　王彦 高婷婷 廉如 陈思思 黄敏 吴彬 陈显久 杨静

　　【摘要】 目的 探讨原核基因重组脂联素球状结构域(gAd)影响血糖水平的机制。方法 采用高糖高脂饮食加1%STZ 35 mg/kg建立T2DM大鼠模型。将造模成功的T2DM大鼠随机分为4组，检测干预前后血糖水平和40 min后大鼠肝、肌肉组织己糖激酶(HK)和丙酮酸激酶(PK)活力。结果 gAd干预40 min时，实验组2、3的血糖低于对照组，干预40 min后，大鼠肝、肌肉组织HK和PK活力升高差异有统计学意义(P<0.05)。结论 gAd急性干预T2DM大鼠可通过增强肝、肌肉组织糖酵解途径降低血糖水平，在治疗T2DM方面可能有良好的应用前景。

　　【关键词】脂联素球状结构域;糖尿病，2型;己糖激酶活力;丙酮酸激酶活力

　　Effect of globular domain of adiponectin on hexokinase and pyruvate kinase vitality in T2DM rat models WANG Yan, GAO Ting-ting, LIAN Ru, et al. Department of Endocrinology, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.

　　Corresponding author: YANG Jing; E-mail: yangjlm@yahoo.com.cn

　　【Abstract】Objective To investigate the mechanism of globular domain of adiponectin (gAd) influencing the plasma glucose level in T2DM rat models. Methods Rat models of T2DM was established by sucrose and fat-enriched diet and intraperitoneal administration of low-dose streptozotocin (STZ,35 mg/kg). The model rats were randomized into four groups. The 0 min, 10 min, 20 min, and 40 min plasma glucose levels after intervention were measured, and the HK and PK vitality in the liver and muscle tissue were detected 40 min after intervention. Results At the time of 40 min of intervention, the plasma glucose level in the gAd intervention groups 2 and 3 was lower than that in the control group. At the time of 40 min after intervention, the increase of K and PK vitality in the liver and muscle tissue of model rats was statistically significant (P<0.05). Conclusion Administration of gAd to T2DM rats reduces the plasma glucose level by enhancing the glycolysis in liver and muscle tissue. It may possess a good prospect in treating T2DM.

　　【Key words】Globular domain of adiponectin; Diabetes mellitus, type 2; Hexokinase vitality; Pyruvate kinase vitality