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赋值传递与方法学比对提高糖化血红蛋白可比性的观察

来自:中国糖尿病杂志  编辑:俞叶夫 包国祥 赵国民|点击数:|2013-04-02

  ·糖尿病临床研究·

  赋值传递与方法学比对提高糖化血红蛋白可比性的观察

  俞叶夫 包国祥 赵国民 杨贵儿

  【摘要】目的 提高不同检测系统测定HbA1c结果的可比性。为实验室间HbA1c结果的互认提供参考。方法 用新鲜混合全血标本赋值传递并作方法学比对,比较赋值传递前后胶乳增强免疫比浊法(LETIA)与高效液相色谱法(HPLC)的可比性。结果 与HPLC法相比LETIA法经赋值传递后在医学决定水平处的偏倚由8.83%下降到0.65%。结论 用新鲜混合全血标本作赋值传递并作方法学比对可提高HbA1c结果的可比性。

  【关键词】糖化血红蛋白;检测系统;方法学;比对;偏倚

  Improvement in the comparability of HbA1c by transferring value-assignment and methodology comparison YU Ye-fu, BAO Guo-xiang, ZHAO Guo-min, et al. Department of Clinical Laboratory, The Affiliated Hospital of Shaoxing University, Shaoxing 312000, China

  【Abstract】 Objective To improve the comparability of HbA1c results from different detecting systems, to provide the valuable reference for the mutual recognition of HbA1c results among medical laboratories. Methods The fresh mixed whole-blood samples were used for transferring value-assignment and methodology comparison in order to compare the comparability of two methods, namely the Latex enhanced turbid-metric Immunoassay (LETIA) and the High performance liquid chromatography (HPLC), before and after transferring value-assignment. Results Compared with HPLC method, after transferring value-assignment, the LETIA method made the bias at medical decision level decreased from 8.83% to 0.65%. Conclusions The comparability of HbA1c results is improved by transferring value-assignment and methodology comparison with the fresh mixed whole-blood samples.

  【Key words】 HbA1c; Detection system; Methodology; Comparison test; Bias

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