【摘要】 目的 研究二甲双胍(MF)对晚期糖基化终末产物(AGEs)诱导的大鼠颅骨成骨细胞增殖、分化、矿化的影响。 方法 分离培养大鼠颅骨成骨细胞，四氮唑蓝比色分析(MTT)法测定细胞增殖，生化法测定碱性磷酸酶(ALP)活性、茜素红S钙染法检测矿化结节形成，盐酸脱钙法检测矿化结节中钙含量。 结果 500μg/ml AGEs抑制成骨细胞增殖、ALP活性、钙化结节形成、钙沉积;给予MF(100～500μmol/L)可不同程度上提高成骨细胞数量和ALP 活性，促进矿化结节形成及钙沉积，减轻AGEs对成骨细胞增殖、ALP活性、钙化结节形成及钙沉积的抑制。 结论 AGEs对原代成骨细胞增殖、分化与矿化产生抑制作用，二甲双胍提高成骨细胞的成骨能力，减轻AGEs对成骨细胞功能的损害。
Metformin ameliorating the deleterious effects of advanced glycation end products on osteoblastic cells ZHEN Dong-hu, TANG Xu-lei, CHENG Jiang-guo, et al. Department of Endocrinology, The First Hospital of Lanzhou University, Lanzhou 730000, China
Corresponding author: TANG Xu-lei, E-mail: email@example.com
【Abstract】Objective To explore the effect of metformin (MF) on proliferation, differentiation, mineralization in the advanced glycation end products (AGEs)-induced rat cranioaural osteoblasts. Methods Rat cranioaural osteoblasts were isolated and cultured. The proliferation of osteoblasts was assayed by MTT method, the activity of alkaline phosphatase (ALP) was measured by biochemical method, the number of mineralized nodules was assessed by Alizarin red S staining, and the calcium deposition in the mineralized nodules was detected by hydrochloric acid decalcification method. Results With 500μg/ml AGEs, the cellular proliferation, ALP activity, number of mineralized nodules, and calcium deposition were reduced significantly. MF(100～500μmol/L) increased the cellular proliferation and ALP activity, and promoted the formation of mineralized nodules and calcium deposition in both control and AGEs groups. Conclusion AGEs inhibits the proliferation, differentiation and mineralization of the primary osteoblasts. MF strengthens the osteogenesis of osteoblasts and relieves the deleterious effect of AGEs on osteoblasts.
【Key words】 Metformin; Advanced glycation end products; Diabetes mellitus; Osteoporosis