【摘要】目的 通过糖代谢紊乱状态患者血清基质细胞衍生因子-1α(stromal cell—derived factor-lα, SDF-1α)与循环内皮祖细胞(endothelial progenitor cells,EPCs)的相关性研究，阐明糖代谢紊乱SDF-1对EPCs的影响。 方法 采集60例正常成人、78例IGT、71例无血管并发症T2DM、60例血管并发症T2DM患者的外周血，以密度梯度离心法获得单个核细胞分离鉴定EPCs，分别测定EPCs的数量、迁移、黏附、增殖、体外血管生成能力，酶联免疫吸附法检测SDF-1α。分析受试者SDF-1、EPCs的差异，观察各组患者SDF-1α与EPCs的数量、迁移、黏附、增殖、体外血管生成能力的相关性。结果 正常、IGT、无血管并发症T2DM、血管并发症T2DM组SDF-1为(6.52±1.04)µg/L、(6.23±1.02)µg/L、(5.98±0.97)µg/L、(5.39±0.85)µg/L ，EPCs(数量、迁移、黏附、增殖、体外血管生成能力)为(53.7±6.7)cell/200、(35.17±4.39)cell/200、(25.98±4.39)cell/200、(17.24±2.15)cell/200，(9.7±2.2)cell/200、(6.27±1.42)cell/200、(4.11±0.93)cell/200、(2.35±0.53)cell/200，(22.9±4.3)cell/200、(17.36±3.26)cell/200、(12.36±2.32)cell/200、(7.73±1.43)cell/200，(0.62±0.04)cell/200、(0.51±0.03)cell/200、(0.38±0.03)cell/200、(0.29±0.02)cell/200，(11.3±1.8)tubules/200、(8.82±1.32)tubules/200、(5.69±0.91)tubules/200、(4.10±0.65)tubules/200(P <0.01)。相关分析结果表明，IGT组SDF-1α只与EPCs的数量呈正相关(P<0.05)，无血管并发症T2DM、血管并发症T2DM组中SDF-1α与EPCs中的数量、迁移、黏附、增殖、体外血管生成能力均呈正相关(P<0.05或<0.01)。结论 糖代谢紊乱对EPCs的影响是通过SDF-1α产生，这对开辟新的T2DM防治途经找到了新的线索。

　　【关键词】葡萄糖代谢障碍;糖尿病，2型;干细胞

Effects of SDF-1α on biological function of EPCs in the patients with glucose metabolism disorders FU Wen-ping. Department of Endocrinology, The Fourth Affiliated Hospital of Nanchang University, Nanchang 330003，China

　　【Abstract】 Objective To clarify the effect of stromal cell-derived factor-1α (SDF-1α) on the biological function of circulating endothelial progenitor cells (EPCs) in the patients with glucose metabolism disorders. Methods The peripheral blood was collected from the 60 normal adults, 78 patients with impaired glucose tolerance, 71 T2DM patients without vascular complications, and 60 T2DM patients with vascular complications. The isolation of mononuclear cells was followed by identification of EPCs with density gradient centrifugation, in which, the amount, migration, adhesion, proliferation, and in vitro angiogenesis were measured respectively. SDF-1α was determined by enzyme-linked immunosorbent assay (ELISA). The differences of the subjects in SDF-1 and EPCs were analyzed. The correlation of SDF-1α with EPCs’ amount, migration, adhesion, proliferation, and angiogenesis in vitro were observed. Results In the normal control group, SDF-1α was 6.52±1.04 µg/L; in the IGT group, it was 6.23±1.02 µg/L; in the T2DM without vascular complications group, it was 5.98±0.97 µg/L; and in the T2DM with vascular complications group, it was 5.39±0.85 µg/L. EPCs’ amount, migration, adhesion, proliferation, and angiogenesis in vitro were 53.7±6.7 cell/200, 35.17±4.39 cell/200, 25.98±4.39 cell/200, 17.24±2.15 cell/200, 9.7±2.2 cell/200, 6.27±1.42 cell/200, 4.11±0.93 cell/200, 2.35±0.53 cell/200, 22.9±4.3 cell/200, 17.36±3.26 cell/200, 12.36±2.32 cell/200, 7.73±1.43 cell/200, 0.62±0.04 cell/200, 0.51±0.03 cell/200, 0.38±0.03 cell/200, 0.29±0.02 cell/200, 11.3±1.8 tubules/200, 8.82±1.32 tubules/200, 5.69±0.91 tubules/200, and 4.10±0.65 tubules/200 (P<0.01). The correlation analysis showed that SDF-1α was positively correlated only with the amount of EPCs in the IGT group (P<0.05), while it was correlated positively with the amount, migration, adhesion, proliferation, and angiogenesis in vitro in the T2DM with and without vascular complications groups (P<0.05 or P<0.01). Conclusion In the patients with glucose metabolism disorders, SDF-1α impairs EPCs. This finding provides a new theoretical basis for making strategy in treatment of T2DM.

　　【Key words】Glucose metabolism disorders; Diabetes mellitus, type 2; Stem cells