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白藜芦醇改善糖尿病血管炎症反应与RAGE信号的相关性研究

来自:中国糖尿病资讯网  编辑:editor|点击数:|2012-09-21

  【摘要】 目的 在DM动物模型基础上,研究白藜芦醇对DM血管损伤的保护效应及可能机制。 方法 STZ单次低剂量(40mg/kg)经股静脉注射,联合高脂饮食,建立DM模型。利用酶底物分析技术检测血液中糖化蛋白Fructosamine的含量,在整体主动脉组织上利用Oil Red检测血管内壁脂肪斑块沉积。利用半定量RT-PCR 技术检测主动脉组织中TNF-α及IL-6的水平。利用Western Blotting技术检测主动脉组织中RAGE表达水平。 结果 RSV持续给药可以减轻DM大鼠血液中Frutosamine的含量,抑制主动脉组织中TNF-α及IL-6的水平,同时抑制RAGE的高表达。RSV也显著减少了DM所致的主动脉内壁脂肪斑块沉积。 结论 RSV可以保护糖尿病导致的血管损伤,这一作用可能和RSV减轻糖基化产物聚集,抑制RAGE信号,减轻血管炎症反应,减少血管壁脂肪斑块沉积有关。

  【关键词】白藜芦醇;糖尿病;糖基化产物及其受体;血管

  Resveratrol protecting the aorta against inflammation reaction associated with RAGE signal in diabetic rats LIU Tao, GONG Zeng Feng, YIN Jie, et al. Department of Pharmaceutical Preparation, Lantai Hospital, the First Hospital of Lanzhou University, Lanzhou 730000; China

  Corresponding author: JING Yu Hong, E-mail: jingyh@lzu.edu.cn

  【Abstract】 Objective To study the protective effect and underlying mechanism of resveratrol treatment on vasculature injury in diabetic rats. Methods Diabetic animal models were established by single injection of STZ (40mg/kg) through femoral vein together with high fat diet. Blood fructosamine was measured by using reaction of enzyme-substrate for four months. Lipid plaques were tested by using Oil red staining in intact aorta. Levels of TNF-α and IL-6 in aorta tissue were analyzed by using semi-quantitative RT-PCR technique. Also, expression of RAGE in aorta was tested by Western blotting method. Results Resveratrol treatment attenuated the amount of blood fructosamine, suppressed the expression of TNF-α and IL-6, and decreased the expression of RAGE in aorta as well as ameliorated the deposition of lipid plaques in inner wall of aorta in diabetic rats. Conclusion Resveratrol treatment can protect the vasculature injured by diabetes. This protective effect may relate with its functions in attenuation of accumulation of advanced glycation end product, inhibition of the RAGE signal, amelioration of the vasculature inflammatory reaction, and reduction of deposition of lipid plaques in diabetic rats.

  【Key words】Resveratrol; Diabetes; Glycation end product and its receptor; Vasculature

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