糖尿病基础研究

　　糜酶对糖尿病大鼠肾脏细胞外基质作用的研究

　　白静 张梅 黄雯 聂晓栋

　　【摘要】 目的 观察糜酶在DM大鼠肾损害过程中的作用。 方法 将大鼠随机分为NC组、DM组、应用糜酶抑制剂的糖尿病(Chy-I)组，Chy-I组予糜酶抑制剂治疗12周。 结果 与DM组比，Chy-I组尿白蛋白/肌酐(UAlb/Cr)明显降低[(0.34±0.15)vs(1.38±0.95)g/μmol,P<0.05],高胆固醇血症得到显著改善[(1.95±0.31)vs(2.31±0.30)mmol/L,P<0.05],纤维粘连蛋白(FN)和Ⅳ型胶原(ColⅣ)基因表达下调(0.53 vs 0.71,P<0.01;0.48 vs 0.58,P<0.01)。 结论 糜酶参与了大鼠糖尿病肾病的发生发展。

　　【关键词】 糜酶;糖尿病肾病;细胞外基质

　　Effect of chymase on the renal fibrosis in the diabetic nephropathic rats BAI Jing, ZHANG Mei, HUANG Wen, et al. Department of Nephropathy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

　　Corresponding author: HUANG Wen, E-mail: huangw@trhos.com

　　【Abstract】 Objective To investigate the effect of chymase on the development of renal lesion in diabetic rats. Methods The rats were randomized into control group, DM group, and Chy-I group. The Chy-I group was treated with chymase inhibitor for 12 weeks. Results Compared with the DM group, the level of urinary albumin/creatinin (UAlb/Cr) was decreased in the Chy-I group [(0.34±0.15) vs (1.38±0.95)g/μmol, P<0.05], the hypercholesterinemia was significantly improved [(1.95±0.31) vs (2.31±0.30)mmol/L, P<0.05], and the gene expression of fibronection (FN) and collagen Ⅳ (ColⅣ) was decreased (0.53 vs 0.71, P<0.01; 0.48 vs 0.58, P<0.01). Conclusion In the diabetic rats, chymase plays an important role in the development of renal fibrosis.

　　【Key Words】 Chymase; Diabetic nephropathy; Fibrosis; Extracellular matrix