·糖尿病分子生物学与遗传学·

　　曹音 刘广军 成金罗 杨科春 袁勇贵

　　基金项目：江苏省卫生厅基金项目(200707)

　　作者单位：213000常州，南京医科大学附属常州第二人民医院神经内科(曹音)，药剂科(刘广军)，内分泌科(成金罗、杨科春);东南大学附属中大医院(袁勇贵)

　　通讯作者：袁勇贵

　　【摘要】目的探讨儿茶酚氧位甲基转移酶(COMT)Val 158 Met多态性与糖尿病伴抑郁的关联研究。方法用聚合酶链反应及聚丙烯酰胺凝胶电泳方法，检测246例糖尿病患者和186名健康体检者(对照组)的COMT基因Val 158 Met多态性。用汉密尔顿抑郁量表(HAMD)评定所有糖尿病患者，HAMD≥17分者为糖尿病伴抑郁组，HAMD<7分为糖尿病不伴抑郁组。结果糖尿病伴抑郁组、糖尿病不伴抑郁组的G等位基因和基因型G/G频率均高于对照组，A等位基因和基因型G/A频率低于对照组(P<0.05)。糖尿病不伴抑郁组中，男性基因型A/A分布频率显著高于女性。糖尿病伴抑郁组中，男性比率显著低于女性。结论COMT基因Val 158 Met多态性与糖尿病伴抑郁症可能存在相关性;男性糖尿病患者较女性可能不易伴发抑郁。

　　【关键词】儿茶酚氧位甲基转移酶;糖尿病，2型;抑郁

　　doi:10.3969/j.issn.1006-6187.2012.01.009

　　Association of polymorphism of catechol-o-methyltransferase with depression in patients with type 2 diabetesCAO Yin, LIU Guang-jun, CHENG Jin-luo, et al. Department of Neurology, The Second Hospital of Changzhou, Changzhou 213000, China

　　Corresponding author: YUAN Yong-gui

　　【Abstract】ObjectiveTo study the association of polymorphism of catechol-o-methyltransferase with depression in patients with type 2 diabetes. MethodsUsing a polymerase chain reaction, val158met polymorphism of COMT was determined in 246 type 2 diabetes and 186 health control subjects. All of patients with type 2 diabetes received HAMD scoring. HAMD rating scale ≥17 represented depression; and <7, no depression. ResultsAllele G and genotype GG frequencies were higher in T2DM both with and without depression group than in control group (all P<0.05) and A allele and G/A genotype frequencies were lower in T2DM both with and without depression group than in control group. A/A genotype frequency was higher in male than in female in T2DM without depression (P<0.05). Prevaleuce of T2DM plus depression was lower in male than in female (P<0.05).Conclusion(1)The polymorphism of COMT gene val158met associates with type 2 diabetes plus depression. (2) Male patients with type 2 diabetes show less incident depression than female patients with type 2 diabetes.

　　【Key words】Catechol-o-methyltransferase; Diabetes mellitus, type 2; Depression