基金项目：国家自然科学基金项目(30971393);天津市科技支撑重点项目(08ZCGYSF01700);天津市卫生局科技基金重点项目(09KZ01);天津医科大学科技基金资助项目(2007ky15,2009ky25)

作者单位：300070天津，天津医科大学代谢病医院，卫生部及天津市激素与发育重点实验室(王倩倩、于珮、孟树优、周赛君、李代清、于德民);天津市眼科医院(陈松)

通讯作者：于德民，E-mail: yudemintij@126.com

结合珠蛋白基因多态性与糖尿病

视网膜病变(二)

王倩倩陈松于珮孟树优周赛君李代清于德民

【摘要】目的

探讨结合珠蛋白(Hp)多态性与2型糖尿病(T2DM)患者糖尿病视网膜病变(DR)发病的关系。方法418例T2DM患者分为3组：糖尿病无视网膜病变(NDR)组、非增生型糖尿病视网膜病变(NPDR)组、增生型糖尿病视网膜病变(PDR)组，另选取100名性别构成、年龄与之相匹配的健康者作为对照(NC)组。采用PCR技术扩增Hp1和Hp2特异目的片段，利用聚丙烯酰胺凝胶电泳技术对Hp分型，分析Hp多态性及各项临床指标与DR的关系。结果NDR组、NPDR组与PDR组两两组间，T2DM组与NC组间，Hp基因型及等位基因频率均无统计学差异(P>0.05)。经Logistic回归分析显示：DM病程、SBP、TC、24h尿白蛋白(24h UAlb)是DR的独立危险因素(P<0.05)，Hp与DR发病无关。结论Hp多态性与天津地区DR发病可能无相关性。

【关键词】结合珠蛋白;多态性，基因;糖尿病，2型;糖尿病视网膜病变

doi:10.3969/j.issn.1006-6187.2011.05.003

Association of haptoglobin gene polymorphism with diabetic retinopathy in type 2 diabetes mellitus (B)

WANG Qian-qian, CHEN Song，YU Pei, et al. The Health Ministry Key Lab of Hormone and Development， Metabolic Disease Hospital， Tianjin Medical University， Tianjin 300070，China

Corresponding author：YU De-min，E-mail：yudemintij@126.com

【Abstract】Objective

To explore the possible correlation between haptoglobin gene polymorphism and diabetic retinopathy in patients with type 2 diabetes mellitus.MethodsA total of 418 type 2 diabetes patients were assigned into 3 groups: non diabetic retinopathy (NDR) group, non-proliferative diabetic retinopathy (NPDR) group and proliferative diabetic retinopathy (PDR) group. The 100 healthy individuals with matched age and sex and serving as control. The Hp genotypes and phenotypes were determined.ResultsThere was no significant difference in Hp genotype and allele frequencies among groups (P>0.05). Logistic regression analysis showed that diabetes duration, systolic blood pressure, total cholesterol and 24-hour urinary albumin were the independent risk factors for diabetic retinopathy (P<0.05). There was no correlation between Hp polymorphism and DR. (P>0.05)ConclusionHp polymorphism may not be associated with the presence of DR in Tianjin population.

【Key words】Haptoglobin; Polymorphism, gene; Diabetes mellitus, type 2; Diabetic retinopathy