基金项目：国家自然科学基金项目(30971393);天津市科技支撑重点项目(08ZCGYSF01700);天津市自然科学基金面上项目(10JCYBJC12000);天津市卫生局科技基金重点项目(09KZ01);天津医科大学科技基金资助项目(2007ky15,2009ky25)

作者单位：300070天津，天津医科大学代谢病医院，卫生部及天津市激素与发育重点实验室(孟树优、李代清、于珮、周赛君、于德民);天津市眼科医院(陈松)

通讯作者：于德民，E-mail:yudemintij@126.com

结合珠蛋白基因多态性与糖尿病

视网膜病变(一)

孟树优陈松李代清于珮周赛君于德民

【摘要】目的

探讨结合珠蛋白(Hp)基因多态性与2型糖尿病(T2DM)患者糖尿病视网膜病变(DR)发病的关系。方法将317例T2DM患者分为糖尿病无视网膜病变(NDR)组和DR组，并选取100名正常人作为对照(NC)组。采用PCR技术扩增Hp1和Hp2特异的目的片段，分析Hp基因型及糖尿病(DM)病程、尿白蛋白(UAlb)等生化指标与DR的关系。结果NDR组、DR组和NC组间Hp等位基因和基因型频率比较，均无统计学差异(P>0.05)。经Logistic回归分析显示，DM病程和24h UAlb是DR的独立危险因素，Hp可能与DR无相关性。结论Hp基因多态性可能与DR无相关性，而DM病程和24h UAlb是DR的独立危险因素。

doi:10.3969/j.issn.1006-6187.2011.05.002

Association of haptoglobin gene polymorphism with diabetic retinopathy in type 2 diabetes mellitus (A)

MENG Shu-you， CHEN Song， LI Dai-qing，et al.Metabolic Disease Hospital， Tianjin Medical University，The Health Ministry Key Lab of Homore and Development，Tianjin 300070，China

Corresponding author：YU De-min，E-mail：yudemintij@126.com

【Abstract】ObjectiveTo investigate the association between haptoglobin(Hp) gene polymorphism and the occurrence of diabetic retinopathy in patients with type 2 diabetes mellitus.MethodsThe 317 type 2 diabetic patients were categorized into two groups: no diabetic retinopathy (NDR) group, diabetic retinopathy (DR) group. In addition, 100 nondiabetic individuals served as control group. The Hp genotypes were determined by PCR-RFLP， Hp genotype frequencies distribution and clinical features were compared among groups.ResultsThere was no significant difference in Hp allele and genotype frequencies distribution among groups. There was no relationship between Hp genotypes and DR (P>0.05). Additionally, logistic regression analysis showed that diabetic duration and 24 hours urinary albumin were independent risk factors for diabetic retinopathy and that the Hp genotype did not have any association with diabetic retinopathy.ConclusionThe Hp polymorphism is not associated with DR. But diabetic duration and 24 hours urinary albumin are independent risk factors for diabetic retinopathy.

【Key words】Haptoglobin; Polymorphism; Diabetes mellitus, type 2; Retinopathy