改变及其与隐形皮肤损害的关系

葛新红张建中张晓鸣汪京峡喻楠夏莉黄继辉施惠娟王建军杨晶

【摘要】目的探讨糖尿病大鼠皮肤的隐形损害现象，以及磷酸化的表皮生长因子受体(P-EGFR)在糖尿病皮肤中的表达与这种隐形损害的关系。方法对比观察不同病期的糖尿病大鼠模型背部和尾巴皮肤组织学改变以及超微结构变化，用免疫组化及蛋白印迹的方法检测P-EGFR在糖尿病大鼠皮肤中的表达。结果糖尿病大鼠皮肤存在组织学和超微结构的改变，P-EGFR在皮肤组织中的表达下降。结论P-EGFR在糖尿病大鼠皮肤组织中表达下调可能与糖尿病皮肤损害的发生有关。

【关键词】糖尿病;皮肤; 受体;表皮生长因子

doi:12.3969/j.issn.1006-6187.2011.05.015

The expression change of phospho-epidermal growth factor receptor and its correlation with the underlying skin damage in diabetic rat

GE Xin-hong, ZHANG Jian-zhong, ZHANG Xiao-ming, et al. Department of Dermatology Affiliated to Ningxia Medical University, Ningxia 750004, China.

Corresponding author: ZHANG Jian-zhong， Email:zhangjz@nxmc.edu.cn

【Abstract】Objective

To detect the underlying skin damage in diabetic rat, and explore the correlation of expression change of p-EGFR(phospho-epidermal growth factor receptor) with the skin damage. MethodsThe changes of the histology and ultrastructure in skin were observed. Immunohistochemical and Western blot techniques were used to detect the expression of EGFR in the rat skin.ResultsThe thickness of the skin decreased and there were ultrastructural changes in the DM rat skin. The expression of phospho-EGFR in the skin of the diabetic rats was lower in 4-week and 8-week DM rats than in control (P<0.05). The expression of P-EGFR in the back and tail skin of the diabetic rats was positively correlated to the thickness of skin(r=0.375 and 0.720，P<0.05 and <0.05). ConclusionThe light and electron microscope-revealed skin damages exist in the diabetes rats, and decreased expression of P-EGFR is correlated with the skin lesions.

【Key words】Diabetes; Skin; Receptor, Epidermal growth factors