·糖尿病中医药治疗研究·

　　梁湘樱 张红杰 朱凌云 罗云 李菁 项阳 张辰宇

　　通讯作者：张辰宇，E-mail: cyzhang@nju.edu.cn

　　作者单位：210093南京大学生命科学院，国家医药重点实验室，江苏省糖尿病中心

　　基金项目：国家自然科学基金资助项目(90813035，30890044);国家基础研究(973)项目(2006CB503909，2004CB518603);863项目(2006AA02A112);江苏省自然科学基金(BK2006714，BK2008021)

　　【摘要】 目的 检测金银花提取物对小鼠肝脏和L02细胞中过氧化物酶体增殖物激活受体γ(PPARγ)共激活因子-1α(PGC-1α)表达及对胰岛素抵抗(IR)的影响。 方法 分别将ob/ob小鼠、C57/B6J小鼠和L02细胞随机分为对照组和实验组，实验组给予金银花提取物，对照组给予生理盐水。给药后检测小鼠肝脏和L02细胞PGC-1α表达。 结果 与对照组相比，实验组ob/ob小鼠给予金银花提取物后肝脏PGC-1α mRNA和蛋白表达下调(P<0.05或P<0.01);而实验组C57/B6J小鼠给药后肝脏PGC-1α mRNA表达与对照组比较无统计学差异。给药5 d后实验组ob/ob小鼠FPG降低，但实验组与对照组组间胰岛素水平无统计学差异，实验组FPG/FIns比值显著降低。胰岛素耐量实验显示金银花提取物改善了ob/ob小鼠的IR。脂肪酸培养L02细胞，给予金银花提取物后PGC-1α mRNA表达下调(P<0.05)，而正常培养L02细胞PGC-1α mRNA表达无变化。 结论 金银花提取物可以下调PGC-1α在肝脏中的病理性高表达，降低血糖，改善IR。

　　【关键词】过氧化物酶体增殖物激活受体γ共激活因子-1α;胰岛素抵抗;金银花;ob/ob小鼠

　　doi:10.3969/j.issn.1006-6187.2011.03.011

　　Effects of extracts of Lonicera japonica Thunb. on hepatic PGC-1α expression and insulin resistance in mouse

　　LIANG Xiang-ying, ZHANG Hong-jie, ZHU Ling-yun, et al.

　　Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China

　　Corresponding author：ZHANG Chen-yu,E-mail: cyzhang@nju.edu.cn

　　【Abstract】 Objective To explore the effects of extracts of Lonicera japonica Thunb. on hepatic PGC-1α expression and insulin resistance in mice and L02 cell. Methods C57/B6J mice were divided into control group (group A)and Lonicera japonica Thunb.-treated group (group B). The ob/ob mice were divided into control group (group C)and Lonicera japonica Thunb.-treated group (group D). L02 cells cultured in normal medium were divided into control group (group a) and Lonicera japonica Thunb.-treated group(group b). L02 cells cultured in medium with FFA (free fatty acid) were divided into control group(group c)and Lonicera japonica Thunb.-treated group (group d). The basal blood glucose and insulin were measured and insulin tolerance test(ITT) was done in ob/ob mice.PGC-1α mRNA and protein expressions were detected by q-RT-PCR and Western blot respectively. Results Hepatic mRNA expression of PGC-1α were significantly decreased in group d versus group c(P<0.05) and in group D versus group C(P<0.05) , while were similar in group b versus group a and in group B versus group A (all P>0.05).Hepatic protein expression of PGC-1α was significantly lower in group D than in group C(P<0.01). Basal blood glucose were significantly lower in group D than in group C (P<0.05), while insulin secretion were similar (P>0.05). Hepatic insulin resistance in group D was improved according to the results of ITT. Conclusions Lonicera japonica Thunb. decreases the pathologically high expression of PGC-1α mRNA in liver and basal blood glucose, and improves insulin resistance .

　　【Key words】Peroxisome proliferators-activated receptor-γ coactivator-1; Insulin resistance; Lonicera japonica thunb; ob/ob mice