·糖尿病基础研究·

　　程庆砾 齐云 马强 赵佳慧 杨光

　　基金项目：国家自然科学基金资助项目(30772296)

　　作者单位：100853北京，解放军总医院老年肾内科

　　【摘要】 目的 观察骨髓干细胞在糖尿病性肾脏疾病表型转移中的作用。 方法 采用链脲佐霉素(STZ)腹腔注射复制糖尿病动物模型，分离糖尿病小鼠和正常小鼠骨髓细胞，经尾静脉分别注入受放射后的小鼠体内作为实验组及对照组，移植后第8周测定各组小鼠空腹血糖和葡萄糖耐量、尿白蛋白、肾小球中α1 IV型胶原和TGF-β1 mRNA的表达及肾脏病理改变。 结果 发现实验组小鼠血糖和葡萄糖耐量基本正常，但尿白蛋白/肌酐比值较对照组高出3.3倍，肾小球体积增大1.2倍，肾小球系膜基质增宽1.98倍，肾内IV型胶原沉积增多，肾小球中α1 IV型胶原 mRNA和TGF-β1 mRNA表达明显增强。 结论 骨髓干细胞在STZ复制的糖尿病小鼠肾脏病变表型转移中具有重要作用。

　　【关键词】小鼠;骨髓干细胞;糖尿病性肾脏疾病;肾脏病理

　　doi:10.3969/j.issn.1006-6187.2011.10.016

　　The role of bone marrow stem cells in the phenotype transfer of diabetic kidney disease

　　CHENG Qing-li, QI Yun，MA Qiang，et al. Department of Geriatric Nephrology, Chinese PLA General Hospital, Beijing 100853, China

　　【Abstract】 Objective To investigate the role of bone marrow (BM) stem cells in the phenotype transfer of diabetic kidney disease. Methods C3H/He female mice with diabetes were induced by intraperitoneal injection of streptozotocin. Normal mice were given a lethall irradiation, then injected via intravenous with 5×106 BM stem cells from diabetic or normal control mice respectively. At 8 weeks after BM transplantation (BMT), the intraperitoneal glucose tolerance test (IPGTT) was performed in the recipient mice. The urinary albumin/creatinine ratio was calculated for each group. Recipient mice were sacrificed at 8 weeks after BMT and the levels of α1 type IV collagen mRNA and TGF-β1 mRNA were measured using real-time RT-PCR. Total glomerular area and the mesangial area were obtained using the MetaMorph image analysis system. Type IV collagen was evaluated in the frozen tissues. Results There was no difference in IPGTT between two group, but the urinary albumin/creatinine ratio was 3.3-fold higher in diabetic mice BMT recipients than in the normal mice BMT recipients (P<0.01). Compared with the normal mice BMT recipients, diabetic mice BMT recipients had markedly increased values of the glomerular area, the ratio of mesangial area over glomerular area and the accumulation of type IV collagen in the glomerular mesangial space. The expression of α1 type IV collagen mRNA and TGF-β1 mRNA in the kidney of the recipients of diabetic BMT were significantly increased (P<0.01). Conclusions The BM stem cells play an important role in the nephrotic phenotype transfer of diabetic kidney disease.

　　【Key words】Mouse; Bone marrow stem cells; Diabetic kidney disease; Kidney pathological lesions