【摘要】  目的    探讨肌肽二肽酶1（CNDP1）基因多态性与中国汉族人群糖尿病慢性肾脏疾病(CKD)的关系。 方法   选取健康对照者（NC）90名、T2DM 患者210例和CKD患者280例（CKD组再分为GFR≥90 ml/min组105例，60 ml/mim≤GFR＜90 ml/min组84例，GFR＜60 ml/min组91例），利用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)行 CNDP1 rs4892247位点多态性检测。 结果   NC、T2DM和CKD组TT、CT、CC 3种基因型频率分别为66.7%、26.7%、6.7%，60.5%、31.9%、7.6%和46.8%43.2%、10.0%；C等位基因频率分别为20.0％、23.6%、31.6%；NC组与CKD组，T2DM组与CKD组基因型和等位基因C的频率分布差异有统计学意义(P<0.017)， Logistic回归分析显示，携带C等位基因的CNDP1与CKD的发生呈正相关（OR=2.634，P<0.05）。   结论  CNDP1rs4892247基因多态性可能与中国汉族人群发生CKD发病风险增高相关。

       【关键词】  肌肽二肽酶1；糖尿病，2型；糖尿病慢性肾脏疾病；基因多态性

       Association analysis of polymorphism in CNDP1 gene and chronic kidney disease in type 2 diabetic Chinese Han population   

   【Abstract】  Objective   To investigate the association between rs4892247 in CNDP1 gene and chronic kidney disease (CKD) in diabetes in Chinese Han population.  Methods    A total of 90 normal controls (NC), 210 paitients with T2DM and 280 patients with CKD were recruited．The CKD subjects were further divided into three groups according to their estimated glomerular filtration rate(eGFR): GFR≥90 ml/min subgroup (n=105)，60 ml/mim≤GFR＜90 ml/min subgroup (n=84)，and GFR＜60 ml/min subgroup (n=91). CNDP1 gene rs4892247 genotype was tested by PCR-RFLP method． Results    The frequencies of TT, CT, CC genotype were 66.7%, 26.7%, 6.7% in NC group, 60.5%, 31.9%, 7.6% in T2DM group and 46.8%, 43.2%, 10.0% in CKD group. There was statistically significant difference in genotypes frequency and CNDP1-C allele frequency in CKD group compare with T2DM group (P<0.017). Logistic regression analysis showed that the CNDP1-C Allele was positively correlated with the occurrence of CKD (OR=2.634,P<0.05).   Conclusion    CNDP1 gene rs4892247 may be associated with CKD in Chinese Han population.

   【Key words】  Carnosine  peptidase 1 （CNDP1）；Diabetes mellitus，type2；Gene polymorphism；Chronic kidney disease (CKD) in diabetes