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2型糖尿病患者血浆中瘦素、胰岛素水平及其microRNA表达的相关性研究

来自:中国糖尿病杂志  编辑:王昱 李杰玉 杜伟鹏|点击数:|2016-03-04

 

   【摘要】 目的  探讨T2DM患者血浆中瘦素、胰岛素水平及其microRNAmiRNA表达的相关性研究。 方法  选取150T2DM患者(T2DM)和150NGT者,比较两组瘦素、胰岛素等水平。瘦素和胰岛素水平采用RIA kit法测定。采用mirVanaTM  

PARISTM Kit提取血浆中RNA,采用TaqMan标准的RT-PCR分析测定miRNA-296miRNA-9的含量。 结果  miRNA-296表达与血浆中瘦素水平有相关性,miRNA-9表达与胰岛素水平有相关性。 结论  T2DM患者血浆中瘦素、胰岛素水平及其miRNA表  达有相关性,但机制尚不明确。 

   【AbstractObjective  To investigate the association between the level of plasma leptin, insulin and their miRNA expression in patients with type 2 diabetes mellitus.  Methods  A total of 300 subjects were enrolled in this study and divided into two groups: T2DM patients as the experimental group (n=150), subjects with normal glucose tolerance as control group (n=150). Plasma leptinand insulin weretested by RIA kit and compared between two groups. Mir Vana TM PARISTM Kit was used for plasma RNA extraction. miRNA-296 and miRNA-9's contentwere measured by the standard TaqMan real-time PCR.  Results   miRNA-296 expression was correlated with plasma leptin levels, and miRNA-9 expression was correlated with plasma insulin levels.   Conclusion  Plasma leptin and insulin levels were correlated with their miRNA expression in patients with T2DM.But the mechanism is unclear.

  【Key wordsDiabetes mellitus,type2; Leptin; Insulin; microRNA(miRNA)

 

 

上一篇:Pax6基因的Arg67Arg与Thr166Thr突变致胰岛素分泌减少所致糖代谢异常与早发2型糖尿病的相关性研究 下一篇:我国汉族糖尿病慢性肾脏疾病与肌肽二肽酶1基因的相关性研究

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