【摘要】  目的    探讨高糖高溶血磷脂胆碱环境对人肾小球系膜细胞(HMCs)产生纤维连接蛋白(Fn)、Ⅳ型胶原(Col Ⅳ)及TGF-β1的影响。 方法    将HMCs分为对照组、TGF-β1中和性抗体组、高糖高脂组、高糖高脂+TGF-β1中和性抗体组，采用qRT-PCR检测TGF-β1 mRNA的表达，通过ELISA法检测细胞上清液中Col IV及Fn含量。  结果  （1）高糖高脂组TGF-β1 mRNA表达水平较对照组升高（P<0.05）；（2）高糖高脂组细胞上清液TGF-β1、Col IV和Fn含量较对照组增加（P <0.05），而这种作用可被TGF-β1中和性抗体所抑制（P <0.05）。结论  高糖高脂可通过促进系膜细胞TGF-β1表达从而诱导ECM的分泌，增加肾纤维化发生的风险。

      【关键词】  高糖高溶血磷脂胆碱；系膜细胞；TGF-β1；细胞外基质

         Effect of high glucose and high lysophosphatidylcholine on the expression of collagen IV, fibronectin and TGF-β1 in mesangial cells  

    【Abstract】 Objective  To investigate the effect of high glucose and high lysophosphatidylcholine(LPC) on the level of fibronectin(Fn), collagen IV(Col Ⅳ), and TGF- beta 1(TGF-β1).  Methods  The human mesangial cells（HMCs）were divided into 4 groups: control group, TGF-β1 neutralizing antibody group, high glucose and LPC group, and high glucose and LPC with TGF-β1 neutralizing antibody group. The mRNA expression of TGF-β1 were detected by qRT-PCR, and the level of Col Ⅳ and Fn in the supernatant were detected by ELISA. Results: 1) The mRNA expression of TGF-β1 was higher in high glucose and LPC group than in control group(P<0.05); 2) TGF-β1, Col IV and Fn in the supernatant were significantly higher in high glucose and LPC group than in control group (P<0.05). However, the effect can be markedly inhibited by TGF-β1 neutralizing antibody(P<0.05). Conclusions High glucose and high LPC could induce the expression of TGF-β1 in HMCs, thus induce the secretion of extra-cellular matrix（ECM）, which increase the potential risk of renal fibrosis.

   【Key words】  High glucose and lysophosphatidylcholine; Mesangial cells; TGF- beta 1; Extracellular matrix