糖尿病基础研究

　　[摘要]　目的　观察血脂变化对OLETF大鼠胰岛素受体底物-1(IRS-1)和含2个SH结构的蛋白酪氨酸磷酸酶(SH-PTP2)蛋白表达的影响。 方法　OLETF大鼠被分为降脂治疗组和对照组。用Western杂交技术检测OLETF大鼠肝脏、脂肪组织内IRS-1及SH-PTP2的蛋白表达水平。 结果　降脂治疗组血糖及血脂水平较对照组下降(P<0. 05)，肝脏、脂肪组织内IRS-1蛋白表达较对照组增加(t=14.61，15.76，P均<0.01)，而SH-PTP2的蛋白表达较对照组减少(t=-3.07，-28.76 P均<0.01)。 结论 降脂治疗可增加OLETF大鼠肝脏、脂肪组织内IRS-1蛋白表达，减少其SH-PTP2蛋白表达，并可能因此影响其胰岛素信号传导，改善其靶组织对胰岛素的敏感性。

　　[关键词]　胰岛素受体底物-1;含2个SH结构的蛋白酪氨酸磷酸酶;糖尿病，2型;OLETF大鼠;非诺贝特;脂代谢

　　[Abstract] Objective To investigate the influence of the changes inlipid on the protein expression of insulin receptor substrate 1 (IRS-1) and protein tyrosine phosphatase with two src-homology 2 (SH-PTP2) in OLETF rats . Methods A total of 14 OLETF rats were randomly divided into lipid-lowering therapy group and control group. The protein expression levels of IRS-1 and SH-PTP2 in liver and adipose tissue were measured by Western blot method. Results The levels of blood glucose and lipid were significantly lower in lipid-lowering therapy group than in control group (P < 0.05);. After lipid-lowering therapy, the protein expression of IRS-1 increased significantly in liver and adipose tissue of OLETF rats (t=14.61, P =0.00; t=15.76, P =0.00, respectively), but the protein expression of SH-PTP2 decreased (t=-3.07, P=0.02; t=-28.76, P=0.00, respectively). Conclusion Lipid-lowering therapy could increase the protein expression of IRS-1 and reduce the expression of SH-PTP2 in liver and adipose tissue,and might therefore affect the insulin signal transductionand improve the insulin sensitivity of target tissues in type 2 diabetic rats.

　　[Key words]　 IRS-1; SH-PTP2; OLETF rats; Type 2 diabetes mellitus; Fenofibrate; Lipid metabolism