·糖尿病基础研究·

　   【摘要】 目的 观察厄贝沙坦对血管生成素样蛋白2(ANGPTL2)在糖尿病大鼠肾脏中表达的影响及作用机制。 方法 50只SD雄性大鼠随机分为正常对照(NC)组10只和实验组40只。实验组给予高糖高脂喂养联合小剂量链脲佐菌素建立T2DM大鼠模型，最终建模成功30只，随机分为糖尿病(DM)组15只和厄贝沙坦(DI)组15只。分别检测8周和12周时体重、BP、肾重、血糖、血肌酐(Scr)、BUN、24 hUAlb水平。镜下观察肾组织病理改变，免疫组织化学染色法、RT-PCR检测肾组织ANGPTL2 mRNA及蛋白表达的变化。 结果 DM组24 hUAlb水平高于DI组和NC组[8周：(7.43±0.36) vs (5.27±0.22) vs (0.74±0.06) mg/24 h;12周：(7.84±0.32) vs (5.11±0.14) vs (0.74±0.08) mg/24 h，P<0.05];DM组8周和12周ANGPTL2 mRNA的表达水平较NC和DI组升高(P<0.05)。 结论 ANGPTL2在糖尿病大鼠肾脏的表达量逐渐升高，而厄贝沙坦可降低其表达。

　　【关键词】 血管生成素样蛋白2;厄贝沙坦;糖尿病肾病

　　【Abstract】 Objective Evaluate the effect of irbesartan on the expression of Angiopoietin-like protein 2 (ANGPTL2) in kidney of diabetics rats and also explore its mechanism. Methods 50 SD male rats were randomly divided into normal control(NC) group and experiment group. Rats in the experiment group was fed with high sugar-fat diet and treated with a low dose streptozocin to build type 2 diabetic models, and then the successful ones (30 rats) were randomly divided into diabetes control (DM) group and irbesartan (DI) group, 15 rats respectively. The weight, blood pressure, blood glucose, serum creatinine(Scr), blood urea nitrogen(BUN), 24 hour urinary albumin(UAlb) level were tested at the 8th and 12th week. Renal histomorphology were recorded and the expression of ANGPTL2 mRNA in renal tissue were detected by immunohistochemisty and real-time PCR. Result 24h UAlb in DM group was higher than in DI and NC groups[8 weeks：(7.43±0.36) vs (5.27±0.22) vs (0.74±0.06) mg/24 h;12 weeks：(7.84±0.32) vs (5.11±0.14) vs (0.74±0.08) mg/24 h，P<0.05];The expression level of ANGPTL2 mRNA in DM group was higher than in NC and DI groups at the 8th and 12th week(P<0.05). Conclusion The expression of ANGPTL2 in kidney of T2DM rats was increased, which could be reduced by irbesartan.

　　【Key words】 Angiopoietin-like protein 2 (ANGPTL2); Irbesartan; Diabetic nephropathy(DN)